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Protein

Acyclic carotenoid 1,2-hydratase

Gene

crtC

Organism
Thiocapsa roseopersicina
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of carotenoids spirilloxanthin. Catalyzes the hydration of lycopene to the corresponding hydroxylated carotenoids 1-HO-lycopene and 1,1'-(HO)2-lycopene. Can also act on geranylgeraniol.2 Publications

Catalytic activityi

1-hydroxy-1,2-dihydrolycopene = lycopene + H2O.1 Publication
1,1'-dihydroxy-1,1',2,2'-tetrahydrolycopene = 1-hydroxy-1,2-dihydrolycopene + H2O.1 Publication

Kineticsi

  1. KM=9.8 µM for lycopene (at pH 8 and at 28 degrees Celsius)1 Publication
  1. Vmax=0.15 nmol/h/mg enzyme with lycopene (at pH 8 and at 28 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8. No activity is detected at pH 4.0-5.0. At higher pH values, it shows rapid decrease in activity, although 50% of the relative activity is still detected at pH 9.0. It retains much residual activity after 30 minutes incubation at pH 4.0-8.0, indicating that CrtC is stable in both slightly alkaline and acid environments.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius. Enzyme activity is significantly lower at 20 degrees Celsius and 40 degrees Celsius.1 Publication

GO - Molecular functioni

  • hydro-lyase activity Source: UniProtKB

GO - Biological processi

  • carotenoid metabolic process Source: UniProtKB
  • chlorophyll biosynthetic process Source: UniProtKB-KW
  • photosynthesis Source: UniProtKB-KW
  • spheroidene biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionLyase
Biological processCarotenoid biosynthesis, Chlorophyll biosynthesis, Photosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Acyclic carotenoid 1,2-hydratase (EC:4.2.1.131)
Alternative name(s):
Hydroxylycopene hydratase
Lycopene hydratase
Gene namesi
Name:crtC
OrganismiThiocapsa roseopersicina
Taxonomic identifieri1058 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeThiocapsa

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004229981 – 405Acyclic carotenoid 1,2-hydrataseAdd BLAST405

Expressioni

Inductioni

Repressed by oxygen.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the CrtC hydratase family.Curated

Phylogenomic databases

KOiK09844.

Sequencei

Sequence statusi: Complete.

Q7X3G5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAAGILTPG ALWAPGPSDT RDERRHDAGR LQPALPGDGR GPLRPGVTRM
60 70 80 90 100
EGLLHASSVA QQGAGALSGR GERASGSRGT DGGHVGTPGG SDPARGPRFD
110 120 130 140 150
LRITPGGYLW WYLDALSDDG DHGLTIIAML GSVFSPYYAW ARRRGNPDPL
160 170 180 190 200
NHCALNVALY GKAGKRWTMT ERGRKALRQA PGRLDIGPSH LTWDGTALTI
210 220 230 240 250
DVNEITAPIP SRVRGRIRVI PAAVNAREFT LDPAERHVWW PIAPISRVEV
260 270 280 290 300
DLEKPALRWS GHGYLDSNRG EEPLEDAFQC WDWSRANTPS GTTMLYDVTA
310 320 330 340 350
RHGTGASLAL RFNASGEVEE FPPPPRVRLP TTGIWRIKRG TQCEAGHQAR
360 370 380 390 400
VVETLEDTPF YARSLVETRL AGETATCVHE SLSLDRFASP VVQLMLPFRM

PRVGG
Length:405
Mass (Da):44,114
Last modified:October 1, 2003 - v1
Checksum:i02B38D5BDFA515EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF528191 Genomic DNA. Translation: AAP59035.1.

Genome annotation databases

KEGGiag:AAP59035.

Similar proteinsi

Entry informationi

Entry nameiCRTC_THIRO
AccessioniPrimary (citable) accession number: Q7X3G5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: October 1, 2003
Last modified: January 20, 2016
This is version 30 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families