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Protein

Sporulation inhibitor sda

Gene

sda

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates a developmental checkpoint inhibiting initiation of sporulation (by preventing phosphorylation of spo0A) in response to defects in the replication initiation machinery. Inhibits autophosphorylation of the histidine protein kinase KinA, forming a molecular barricade that prevents productive interaction between the ATP-binding site in the catalytic domain and the phosphorylatable His in the phosphotransfer domain of KinA. Probably also inhibits the activity of KinB, but has relatively little effect on KinC. Has at least one target in vivo in addition to KinA as sda does not require KinA to inhibit sporulation.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei25Important for interaction with KinACurated1
Sitei27Important for interaction with KinACurated1
Sitei31Important for interaction with KinACurated1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionProtein kinase inhibitor
Biological processSporulation

Enzyme and pathway databases

BioCyciBSUB:BSU25690-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation inhibitor sda
Alternative name(s):
Histidine kinase KinA inhibitor
Gene namesi
Name:sda
Ordered Locus Names:BSU25690
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi25M → A or I: Partial loss of ability to inhibit KinA autophosphorylation. 1 Publication1
Mutagenesisi27L → A or E: Complete loss of ability to inhibit KinA autophosphorylation. 1 Publication1
Mutagenesisi31F → H: Complete loss of ability to inhibit KinA autophosphorylation. 1 Publication1
Mutagenesisi34L → A: Partial loss of ability to inhibit KinA autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000222881 – 52Sporulation inhibitor sdaAdd BLAST52

Proteomic databases

PaxDbiQ7WY62
PRIDEiQ7WY62

Interactioni

Subunit structurei

Forms a stable heterotetramer with KinA (comprising two molecules of each protein), by binding to the KinA dimerization/phosphotransfer domain.

Binary interactionsi

WithEntry#Exp.IntActNotes
kinAP164976EBI-6405714,EBI-6405707

Protein-protein interaction databases

IntActiQ7WY62, 1 interactor
STRINGi224308.Bsubs1_010100014046

Structurei

Secondary structure

152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 24Combined sources13
Helixi29 – 43Combined sources15
Turni45 – 49Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PV0NMR-A7-52[»]
3FYRX-ray1.97A/B/C7-52[»]
ProteinModelPortaliQ7WY62
SMRiQ7WY62
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7WY62

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000262075
KOiK06371
OMAiDLLMETY

Family and domain databases

Gene3Di1.10.287.1100, 1 hit
InterProiView protein in InterPro
IPR015064 Sda
IPR036916 Sda_sf
PfamiView protein in Pfam
PF08970 Sda, 1 hit
SUPFAMiSSF100985 SSF100985, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q7WY62-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MNWVPSMRKL SDELLIESYF KATEMNLNRD FIELIENEIK RRSLGHIISV

SS
Length:52
Mass (Da):6,155
Last modified:October 1, 2003 - v1
Checksum:iBCBDECF0E0203CAE
GO
Isoform 2 (identifier: Q7WY62-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Note: No experimental confirmation available. Seems to be the major isoform.
Show »
Length:46
Mass (Da):5,440
Checksum:i1E9AE14098D07C69
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0187901 – 6Missing in isoform 2. Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAE01463.1
RefSeqiWP_010886571.1, NC_000964.3
YP_054588.1, NC_000964.3 [Q7WY62-1]

Genome annotation databases

EnsemblBacteriaiCAE01463; CAE01463; BSU25690
GeneIDi2914223
KEGGibsu:BSU25690
PATRICifig|224308.43.peg.2679

Keywords - Coding sequence diversityi

Alternative initiation

Entry informationi

Entry nameiSDA_BACSU
AccessioniPrimary (citable) accession number: Q7WY62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: October 1, 2003
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health