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Protein

Beta-N-acetylhexosaminidase

Gene

hex20

Organism
Cellulomonas fimi
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of beta-N-acetylglucosaminides and beta-N-acetylgalactosaminides. Also catalyzes the hydrolysis of N-acetylchitooligomers. May be involved in chitin degradation. It is not able to cleave beta-glucosides.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Kineticsi

There is a 4-fold greater activity on 4'-nitrophenyl beta-N-acetyl-D-glucosaminide than on 4'-nitrophenyl beta-N-acetyl-D-galactosaminide.

  1. KM=53 µM for 4'-nitrophenyl beta-N-acetyl-D-glucosaminide1 Publication
  2. KM=66 µM for 4'-nitrophenyl beta-N-acetyl-D-galactosaminide1 Publication

    pH dependencei

    Optimum pH is 7.3-8.7 with 4'-nitrophenyl beta-N-acetyl-D-glucosaminide as substrate. Rapidly inactivated above pH 9.5 and stable from pH 6.0 to 9.5.1 Publication

    Pathway:ichitin degradation

    This protein is involved in the pathway chitin degradation, which is part of Glycan degradation.
    View all proteins of this organism that are known to be involved in the pathway chitin degradation and in Glycan degradation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei298 – 2981Proton donorBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

    Enzyme and pathway databases

    SABIO-RKQ7WUL4.
    UniPathwayiUPA00349.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-N-acetylhexosaminidase (EC:3.2.1.52)
    Alternative name(s):
    Beta-N-acetylgalactosaminidase
    Beta-N-acetylglucosaminidase
    Hex20
    Gene namesi
    Name:hex20
    Synonyms:hex20A
    OrganismiCellulomonas fimi
    Taxonomic identifieri1708 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaMicrococcalesCellulomonadaceaeCellulomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 496496Beta-N-acetylhexosaminidasePRO_0000252455Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi590998.Celf_3081.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7WUL4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 20 family.Curated

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProiIPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR015882. HEX_bac_N.
    [Graphical view]
    PfamiPF00728. Glyco_hydro_20. 1 hit.
    PF02838. Glyco_hydro_20b. 1 hit.
    [Graphical view]
    PRINTSiPR00738. GLHYDRLASE20.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7WUL4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPDVAVIPRP VLLETTDGPP FVLTAATILV VDSAPELVAV GVLAADLLGR
    60 70 80 90 100
    LSGRPVEVRY TEGGAPSVVR LRLSEDLPAG DEAYRLVVSE HRVDIDARSA
    110 120 130 140 150
    AGLVRAVVTL RQTVSSLGDG TLTVPALRVE DHPRYAWRGL SIDVARHFFT
    160 170 180 190 200
    VDDLKAIIGL LAHYKLNVLH LHLTDDQGWR VHLPSRPHLT RASAGTSVGG
    210 220 230 240 250
    GPGGFYNPAQ LAEIVVARAA RGIRVVPEID VPGHVNAATH AYGDLTPSGE
    260 270 280 290 300
    PTDVYTGIEV GFSRLHDDLP ATRPFLRDVF TDLAAMTPGE YVHIGGDEVL
    310 320 330 340 350
    TMDHDKYARL VGYAASVVRD AGKKVVGWQE ISSTPLEPGT VVQYWDINAD
    360 370 380 390 400
    PAPFVAAAQA GAHVLMSPGS RAYLDMKYDA TTELGLEWAG HIELRDAYDW
    410 420 430 440 450
    EPSTLIPGVP PESVIGVEAA VWTETLTDLG ELTSMLLPRL AAVAEVAWTA
    460 470 480 490
    PQDRDWDDFS GRVAQHAPFW DRVGFRWHAS PQVSWPGPGS APGAAF
    Length:496
    Mass (Da):53,326
    Last modified:October 1, 2003 - v1
    Checksum:i7D514C614991706C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF478459 Genomic DNA. Translation: AAQ05800.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF478459 Genomic DNA. Translation: AAQ05800.1.

    3D structure databases

    ProteinModelPortaliQ7WUL4.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi590998.Celf_3081.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    UniPathwayiUPA00349.
    SABIO-RKQ7WUL4.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProiIPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR015882. HEX_bac_N.
    [Graphical view]
    PfamiPF00728. Glyco_hydro_20. 1 hit.
    PF02838. Glyco_hydro_20b. 1 hit.
    [Graphical view]
    PRINTSiPR00738. GLHYDRLASE20.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. "Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi."
      Mayer C., Vocadlo D.J., Mah M., Rupitz K., Stoll D., Warren R.A.J., Withers S.G.
      FEBS J. 273:2929-2941(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiHEX20_CELFI
    AccessioniPrimary (citable) accession number: Q7WUL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: October 1, 2003
    Last modified: June 24, 2015
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The reaction mechanism involves an oxazolinium ion intermediate.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.