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Q7WUL4

- HEX20_CELFI

UniProt

Q7WUL4 - HEX20_CELFI

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Protein
Beta-N-acetylhexosaminidase
Gene
hex20, hex20A
Organism
Cellulomonas fimi
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of beta-N-acetylglucosaminides and beta-N-acetylgalactosaminides. Also catalyzes the hydrolysis of N-acetylchitooligomers. May be involved in chitin degradation. It is not able to cleave beta-glucosides.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Kineticsi

There is a 4-fold greater activity on 4'-nitrophenyl beta-N-acetyl-D-glucosaminide than on 4'-nitrophenyl beta-N-acetyl-D-galactosaminide.

  1. KM=53 µM for 4'-nitrophenyl beta-N-acetyl-D-glucosaminide1 Publication
  2. KM=66 µM for 4'-nitrophenyl beta-N-acetyl-D-galactosaminide

pH dependencei

Optimum pH is 7.3-8.7 with 4'-nitrophenyl beta-N-acetyl-D-glucosaminide as substrate. Rapidly inactivated above pH 9.5 and stable from pH 6.0 to 9.5.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei298 – 2981Proton donor By similarity

GO - Molecular functioni

  1. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. chitin catabolic process Source: UniProtKB-UniPathway
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Enzyme and pathway databases

SABIO-RKQ7WUL4.
UniPathwayiUPA00349.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-N-acetylhexosaminidase (EC:3.2.1.52)
Alternative name(s):
Beta-N-acetylgalactosaminidase
Beta-N-acetylglucosaminidase
Hex20
Gene namesi
Name:hex20
Synonyms:hex20A
OrganismiCellulomonas fimi
Taxonomic identifieri1708 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Beta-N-acetylhexosaminidase
PRO_0000252455Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ7WUL4.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF02838. Glyco_hydro_20b. 1 hit.
[Graphical view]
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7WUL4-1 [UniParc]FASTAAdd to Basket

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MPDVAVIPRP VLLETTDGPP FVLTAATILV VDSAPELVAV GVLAADLLGR    50
LSGRPVEVRY TEGGAPSVVR LRLSEDLPAG DEAYRLVVSE HRVDIDARSA 100
AGLVRAVVTL RQTVSSLGDG TLTVPALRVE DHPRYAWRGL SIDVARHFFT 150
VDDLKAIIGL LAHYKLNVLH LHLTDDQGWR VHLPSRPHLT RASAGTSVGG 200
GPGGFYNPAQ LAEIVVARAA RGIRVVPEID VPGHVNAATH AYGDLTPSGE 250
PTDVYTGIEV GFSRLHDDLP ATRPFLRDVF TDLAAMTPGE YVHIGGDEVL 300
TMDHDKYARL VGYAASVVRD AGKKVVGWQE ISSTPLEPGT VVQYWDINAD 350
PAPFVAAAQA GAHVLMSPGS RAYLDMKYDA TTELGLEWAG HIELRDAYDW 400
EPSTLIPGVP PESVIGVEAA VWTETLTDLG ELTSMLLPRL AAVAEVAWTA 450
PQDRDWDDFS GRVAQHAPFW DRVGFRWHAS PQVSWPGPGS APGAAF 496
Length:496
Mass (Da):53,326
Last modified:October 1, 2003 - v1
Checksum:i7D514C614991706C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF478459 Genomic DNA. Translation: AAQ05800.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF478459 Genomic DNA. Translation: AAQ05800.1 .

3D structure databases

ProteinModelPortali Q7WUL4.
ModBasei Search...

Protein family/group databases

CAZyi GH20. Glycoside Hydrolase Family 20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00349 .
SABIO-RK Q7WUL4.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProi IPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
[Graphical view ]
Pfami PF00728. Glyco_hydro_20. 1 hit.
PF02838. Glyco_hydro_20b. 1 hit.
[Graphical view ]
PRINTSi PR00738. GLHYDRLASE20.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi."
    Mayer C., Vocadlo D.J., Mah M., Rupitz K., Stoll D., Warren R.A.J., Withers S.G.
    FEBS J. 273:2929-2941(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiHEX20_CELFI
AccessioniPrimary (citable) accession number: Q7WUL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 1, 2003
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The reaction mechanism involves an oxazolinium ion intermediate.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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