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Q7WUL3

- NAG3_CELFI

UniProt

Q7WUL3 - NAG3_CELFI

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Protein

Beta-N-acetylglucosaminidase/beta-glucosidase

Gene
nag3, nag3A
Organism
Cellulomonas fimi
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of beta-N-acetyl-D-glucosaminides and beta-D-glucosides. Might be involved in the degradation of glucuronic acid-containing glycosaminoglycans such as hyaluronic acid.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication

Kineticsi

The catalytic efficiencies against 4'-nitrophenyl beta-N-acetyl-D-glucosaminide and 4'-nitrophenyl beta-D-glucopyranoside are similar.

  1. KM=2.7 mM for 4'-nitrophenyl beta-N-acetyl-D-glucosaminide1 Publication

pH dependencei

Optimum pH is 7.3 with 4'-nitrophenyl beta-D-glucopyranoside as substrate. Precipitates below pH 6 and stable from pH 6.8 to 8.4.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei283 – 2831Nucleophile By similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC
  2. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

SABIO-RKQ7WUL3.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-N-acetylglucosaminidase/beta-glucosidase (EC:3.2.1.21, EC:3.2.1.52)
Alternative name(s):
3-beta-N-acetyl-D-glucosaminidase/beta-D-glucosidase
Nag3
Gene namesi
Name:nag3
Synonyms:nag3A
OrganismiCellulomonas fimi
Taxonomic identifieri1708 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Beta-N-acetylglucosaminidase/beta-glucosidasePRO_0000252454Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ7WUL3.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
InterProiIPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7WUL3-1 [UniParc]FASTAAdd to Basket

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MIDLTAAPFS LDDDGIAWVR TTLAEMGEDE KLGQLFCLIT YTSDPEYLGY    50
LTRGLHVGGV MLRTMTAADA AATVTTLQST ATVPLLISAN LEGGASQTVQ 100
EATHVGSNMA LAATGSTDHV RRAATVIGRE ARALGINWAF TPVVDIDLNF 150
RNPITNTRTF GADAATVAAM GAEYVEAIQA QGLAASAKHF PGDGVDERDQ 200
HLLASVNTMS VEEWDDSFGV VYRAAIAAGV KTVMVGHIML PAYSRALRPG 250
VADRDILPGV VAEELLNDLL RDRLGFNGLV VSDSTTMAGL ASVLPRSQAV 300
PRVIAAGCDM FLFTKNLDED FGYMRAGIRD GVITPERLDE AVTRILALKA 350
SLGLHRGTNL PAQGAAGVLA DPDHSATARE VAASSITLVK EEPGVLPITR 400
ERYPRVLVYD LQNGGSPIGQ GARAGAVEQF VDALVEAGHD VTRFEPGGGW 450
EGMAAPTTDV TERHDLVLYL ANLSTRSNQT VVRIEWAEPM GANVPAYVHS 500
VPTVFVSFEN PYHLFDVPRV RTLINTYGSS PVVLETLLAA LQGKAPFAGS 550
SPVDAFCGQW DTHL 564
Length:564
Mass (Da):59,993
Last modified:October 1, 2003 - v1
Checksum:i9B1428A4561F71D3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF478460 Genomic DNA. Translation: AAQ05801.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF478460 Genomic DNA. Translation: AAQ05801.1 .

3D structure databases

ProteinModelPortali Q7WUL3.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK Q7WUL3.

Family and domain databases

Gene3Di 3.20.20.300. 1 hit.
InterProi IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00933. Glyco_hydro_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi."
    Mayer C., Vocadlo D.J., Mah M., Rupitz K., Stoll D., Warren R.A.J., Withers S.G.
    FEBS J. 273:2929-2941(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-11, FUNCTION, CATALYTIC ACTIVITY, REACTION MECHANISM, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiNAG3_CELFI
AccessioniPrimary (citable) accession number: Q7WUL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 1, 2003
Last modified: October 16, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Catalyzes hydrolysis by a double-displacement mechanism via a covalent glycosyl-enzyme intermediate, involving the participation of a catalytic nucleophilic group in the enzyme active site.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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