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Q7WUL3

- NAG3_CELFI

UniProt

Q7WUL3 - NAG3_CELFI

Protein

Beta-N-acetylglucosaminidase/beta-glucosidase

Gene

nag3

Organism
Cellulomonas fimi
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the cleavage of beta-N-acetyl-D-glucosaminides and beta-D-glucosides. Might be involved in the degradation of glucuronic acid-containing glycosaminoglycans such as hyaluronic acid.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication
    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication

    Kineticsi

    The catalytic efficiencies against 4'-nitrophenyl beta-N-acetyl-D-glucosaminide and 4'-nitrophenyl beta-D-glucopyranoside are similar.

    1. KM=2.7 mM for 4'-nitrophenyl beta-N-acetyl-D-glucosaminide1 Publication

    pH dependencei

    Optimum pH is 7.3 with 4'-nitrophenyl beta-D-glucopyranoside as substrate. Precipitates below pH 6 and stable from pH 6.8 to 8.4.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei283 – 2831NucleophileBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB-EC
    2. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Enzyme and pathway databases

    SABIO-RKQ7WUL3.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-N-acetylglucosaminidase/beta-glucosidase (EC:3.2.1.21, EC:3.2.1.52)
    Alternative name(s):
    3-beta-N-acetyl-D-glucosaminidase/beta-D-glucosidase
    Nag3
    Gene namesi
    Name:nag3
    Synonyms:nag3A
    OrganismiCellulomonas fimi
    Taxonomic identifieri1708 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 564564Beta-N-acetylglucosaminidase/beta-glucosidasePRO_0000252454Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ7WUL3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 3 family.Curated

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    InterProiIPR019800. Glyco_hydro_3_AS.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00933. Glyco_hydro_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7WUL3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIDLTAAPFS LDDDGIAWVR TTLAEMGEDE KLGQLFCLIT YTSDPEYLGY    50
    LTRGLHVGGV MLRTMTAADA AATVTTLQST ATVPLLISAN LEGGASQTVQ 100
    EATHVGSNMA LAATGSTDHV RRAATVIGRE ARALGINWAF TPVVDIDLNF 150
    RNPITNTRTF GADAATVAAM GAEYVEAIQA QGLAASAKHF PGDGVDERDQ 200
    HLLASVNTMS VEEWDDSFGV VYRAAIAAGV KTVMVGHIML PAYSRALRPG 250
    VADRDILPGV VAEELLNDLL RDRLGFNGLV VSDSTTMAGL ASVLPRSQAV 300
    PRVIAAGCDM FLFTKNLDED FGYMRAGIRD GVITPERLDE AVTRILALKA 350
    SLGLHRGTNL PAQGAAGVLA DPDHSATARE VAASSITLVK EEPGVLPITR 400
    ERYPRVLVYD LQNGGSPIGQ GARAGAVEQF VDALVEAGHD VTRFEPGGGW 450
    EGMAAPTTDV TERHDLVLYL ANLSTRSNQT VVRIEWAEPM GANVPAYVHS 500
    VPTVFVSFEN PYHLFDVPRV RTLINTYGSS PVVLETLLAA LQGKAPFAGS 550
    SPVDAFCGQW DTHL 564
    Length:564
    Mass (Da):59,993
    Last modified:October 1, 2003 - v1
    Checksum:i9B1428A4561F71D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF478460 Genomic DNA. Translation: AAQ05801.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF478460 Genomic DNA. Translation: AAQ05801.1 .

    3D structure databases

    ProteinModelPortali Q7WUL3.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH3. Glycoside Hydrolase Family 3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK Q7WUL3.

    Family and domain databases

    Gene3Di 3.20.20.300. 1 hit.
    InterProi IPR019800. Glyco_hydro_3_AS.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00933. Glyco_hydro_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00775. GLYCOSYL_HYDROL_F3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi."
      Mayer C., Vocadlo D.J., Mah M., Rupitz K., Stoll D., Warren R.A.J., Withers S.G.
      FEBS J. 273:2929-2941(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-11, FUNCTION, CATALYTIC ACTIVITY, REACTION MECHANISM, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiNAG3_CELFI
    AccessioniPrimary (citable) accession number: Q7WUL3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Catalyzes hydrolysis by a double-displacement mechanism via a covalent glycosyl-enzyme intermediate, involving the participation of a catalytic nucleophilic group in the enzyme active site.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3