Reviewed, UniProtKB/Swiss-Prot Q7WTJ6 (DMPL_ACICA)

Names and origin

Protein names

Recommended name:
    Phenol hydroxylase P1 protein
    EC=1.14.13.7
Alternative name(s):
    Phenol 2-monooxygenase P1 component

Gene names

Name:

mphL

Organism

Acinetobacter calcoaceticus

Taxonomic identifier

471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catabolizes phenol, and some of its methylated derivatives. P1 is required for growth on phenol, and for in vitro phenol hydroxylase activity By similarity. UniProtKB P19730
Catalytic activity	Phenol + NADPH + O2 = catechol + NADP+ + H2O. UniProtKB P19730
Cofactor	FAD By similarity. Fe2+ By similarity.
Pathway	Aromatic compound metabolism; phenol degradation.
Subunit structure	The multicomponent enzyme phenol hydroxylase is formed by P0, P1, P2, P3, P4 and P5 polypeptides By similarity. UniProtKB P19730
Induction	By phenol.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein Iron NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aerobic phenol catabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenol 2-monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 332	331	Phenol hydroxylase P1 protein		PRO_0000079941

Sequences

	Sequence		Length	Mass (Da)
	Q7WTJ6-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FCAE249C720A0BA6	FASTA	332	38,226
	10 20 30 40 50 60 MTLEIKTSNV EPIRQNYAYI ERRFGSKPAT RYQEVSFDVQ AETNFHYRPL WKPEKTLNDK 70 80 90 100 110 120 THTALQMQDW YAFKDPRQFY YGTYVQHRAR LQDTAESNFA FFEKRQLAEH LSNEVKAKVI 130 140 150 160 170 180 ECLLPFRHVE QTANLHMMSG SAYGYGTVLT QACIYAAMDH LGIAQYISRI GLALDGNSGD 190 200 210 220 230 240 SLQQAKQAWM QHPAWQGLRR LCEESLTEQD YFKLFLLQNL VIDGFVTELV YQQFDQWLVS 250 260 270 280 290 300 QNARDLAMLT EFMKDTLGDL RKWSDTVIKT AAAESDHNKQ LLNEWFTESL AQVKAAFTPW 310 320 330 ATAALTVDAV DQAEQAVIER AKKLGLQPLT NA

References

[1]	"Genetic organization of genes encoding phenol hydroxylase, benzoate 1,2-dioxygenase alpha subunit and its regulatory proteins in Acinetobacter calcoaceticus PHEA-2." Xu Y., Chen M., Zhang W., Lin M. Curr. Microbiol. 46:235-240(2003) [PubMed: 12732969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PHEA-2.
[2]	"Growth on phenol at chemostress levels amplifies the expression of the phenol degradation pathway in Acinetobacter calcoaceticus." Benndorf D., Loffhagen N., Hartig C., Babel W. Eng. Life Sci. 4:38-42(2004) Cited for: PROTEIN SEQUENCE OF 2-16. Strain: 69-V.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ564846 Genomic DNA. Translation: CAD92312.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.7. 412.
Family and domain databases
InterPro	IPR012078. MP_mOase_hydro. IPR003430. Phenol_Hydrox. IPR012348. Ribncl_red_rel. [Graphical view]
Gene3D	G3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.
Pfam	PF02332. Phenol_Hydrox. 1 hit. [Graphical view]
PIRSF	PIRSF000040. MMOH_comp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DMPL_ACICA

Accession

Primary (citable) accession number: Q7WTJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	January 23, 2007
Last modified:	January 20, 2009
This is version 30 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)