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Protein

Phenol hydroxylase P5 protein

Gene

mphP

Organism
Acinetobacter calcoaceticus (strain PHEA-2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catabolizes phenol, and some of its methylated derivatives. P5 is required for growth on phenol, and for in vitro phenol hydroxylase activity (By similarity).By similarity
Probable electron transfer from NADPH, via FAD and the 2Fe-2S center, to the oxygenase activity site of the enzyme.By similarity

Catalytic activityi

Phenol + NADPH + O2 = catechol + NADP+ + H2O.By similarity

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster.By similarity

Pathwayi: phenol degradation

This protein is involved in the pathway phenol degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenol degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi42Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi45Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi77Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processAromatic hydrocarbons catabolism, Electron transport, Transport
Ligand2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciACAL871585:G1GRU-479-MONOMER
UniPathwayiUPA00728

Protein family/group databases

TCDBi5.B.1.3.2 the phagocyte (gp91(phox)) nadph oxidase family

Names & Taxonomyi

Protein namesi
Recommended name:
Phenol hydroxylase P5 protein (EC:1.14.13.7)
Alternative name(s):
Phenol 2-monooxygenase P5 component
Gene namesi
Name:mphP
Ordered Locus Names:BDGL_000470
OrganismiAcinetobacter calcoaceticus (strain PHEA-2)
Taxonomic identifieri871585 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex
Proteomesi
  • UP000007477 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001894062 – 353Phenol hydroxylase P5 proteinAdd BLAST352

Expressioni

Inductioni

By phenol.Curated1 Publication

Interactioni

Subunit structurei

The multicomponent enzyme phenol hydroxylase is formed by P0, P1, P2, P3, P4 and P5 polypeptides.By similarity

Protein-protein interaction databases

STRINGi871585.BDGL_000470

Structurei

3D structure databases

ProteinModelPortaliQ7WTJ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 932Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST91
Domaini102 – 201FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST100

Phylogenomic databases

eggNOGiENOG4108JA0 Bacteria
COG0543 LUCA
KOiK16246
OMAiYMTLERR

Family and domain databases

CDDicd00207 fer2, 1 hit
Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR012675 Beta-grasp_dom_sf
IPR017927 Fd_Rdtase_FAD-bd
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR001221 Phe_hydroxylase
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00111 Fer2, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00410 PHEHYDRXLASE
SUPFAMiSSF54292 SSF54292, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7WTJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYQVTIEPI GTTIEVEEDQ TILDAALRQG VWLPFACGHG TCGTCKVQVT
60 70 80 90 100
DGFYDVGEAS PFALMDIERD ENKVLACCCK PQSDMVIEAD VDEDPDFLGH
110 120 130 140 150
LVQDYQATVI EIKDLSPTIK GIRLQLDRPI EFQAGQYINV QFPNIEGTRA
160 170 180 190 200
FSIANSPSEV GIVELHIRKV EGGAATTYVH EQLATGDQLD ISGPYGQFFV
210 220 230 240 250
RKSDDQNAIF IAGGSGLSSP QSMILDLLES GDSRTIYLFQ GARDLAELYN
260 270 280 290 300
RELFEQLVKD YPNFRYIPAL NAPKPEDQWT GFTGFVHEAV ADYFENRCGG
310 320 330 340 350
HKAYLCGPPI MIDSAISTLM QSRLFERDIH TERFLSAADG AAGQSRSALF

KHI
Length:353
Mass (Da):39,038
Last modified:January 23, 2007 - v3
Checksum:iCFA97CFD40458549
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ564846 Genomic DNA Translation: CAD92316.1
CP002177 Genomic DNA Translation: ADY81056.1
RefSeqiWP_014206216.1, NC_016603.1
YP_004994738.1, NC_016603.1

Genome annotation databases

EnsemblBacteriaiADY81056; ADY81056; BDGL_000470
GeneIDi11638648
KEGGiacc:BDGL_000470
PATRICifig|871585.3.peg.467

Similar proteinsi

Entry informationi

Entry nameiDMPP_ACICP
AccessioniPrimary (citable) accession number: Q7WTJ2
Secondary accession number(s): F0KIB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 78 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health