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Protein

Phenol hydroxylase P5 protein

Gene

mphP

Organism
Acinetobacter calcoaceticus (strain PHEA-2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catabolizes phenol, and some of its methylated derivatives. P5 is required for growth on phenol, and for in vitro phenol hydroxylase activity (By similarity).By similarity
Probable electron transfer from NADPH, via FAD and the 2Fe-2S center, to the oxygenase activity site of the enzyme.By similarity

Catalytic activityi

Phenol + NADPH + O2 = catechol + NADP+ + H2O.By similarity

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster.By similarity

Pathwayi: phenol degradation

This protein is involved in the pathway phenol degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenol degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi37 – 371Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi42 – 421Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi45 – 451Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi77 – 771Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism, Electron transport, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00728.

Protein family/group databases

TCDBi5.B.1.8.2. the phagocyte (gp91(phox)) nadph oxidase family.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenol hydroxylase P5 protein (EC:1.14.13.7)
Alternative name(s):
Phenol 2-monooxygenase P5 component
Gene namesi
Name:mphP
Ordered Locus Names:BDGL_000470
OrganismiAcinetobacter calcoaceticus (strain PHEA-2)
Taxonomic identifieri871585 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex
Proteomesi
  • UP000007477 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 353352Phenol hydroxylase P5 proteinPRO_0000189406Add
BLAST

Expressioni

Inductioni

By phenol.Curated1 Publication

Interactioni

Subunit structurei

The multicomponent enzyme phenol hydroxylase is formed by P0, P1, P2, P3, P4 and P5 polypeptides.By similarity

Protein-protein interaction databases

STRINGi871585.BDGL_000470.

Structurei

3D structure databases

ProteinModelPortaliQ7WTJ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 93912Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini102 – 201100FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108JA0. Bacteria.
COG0543. LUCA.
KOiK16246.
OMAiYMTLERR.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR001221. Phe_hydroxylase.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00410. PHEHYDRXLASE.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7WTJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYQVTIEPI GTTIEVEEDQ TILDAALRQG VWLPFACGHG TCGTCKVQVT
60 70 80 90 100
DGFYDVGEAS PFALMDIERD ENKVLACCCK PQSDMVIEAD VDEDPDFLGH
110 120 130 140 150
LVQDYQATVI EIKDLSPTIK GIRLQLDRPI EFQAGQYINV QFPNIEGTRA
160 170 180 190 200
FSIANSPSEV GIVELHIRKV EGGAATTYVH EQLATGDQLD ISGPYGQFFV
210 220 230 240 250
RKSDDQNAIF IAGGSGLSSP QSMILDLLES GDSRTIYLFQ GARDLAELYN
260 270 280 290 300
RELFEQLVKD YPNFRYIPAL NAPKPEDQWT GFTGFVHEAV ADYFENRCGG
310 320 330 340 350
HKAYLCGPPI MIDSAISTLM QSRLFERDIH TERFLSAADG AAGQSRSALF

KHI
Length:353
Mass (Da):39,038
Last modified:January 23, 2007 - v3
Checksum:iCFA97CFD40458549
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ564846 Genomic DNA. Translation: CAD92316.1.
CP002177 Genomic DNA. Translation: ADY81056.1.
RefSeqiWP_014206216.1. NC_016603.1.
YP_004994738.1. NC_016603.1.

Genome annotation databases

EnsemblBacteriaiADY81056; ADY81056; BDGL_000470.
GeneIDi11638648.
KEGGiacc:BDGL_000470.
PATRICi47078982. VBIAciCal168233_0467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ564846 Genomic DNA. Translation: CAD92316.1.
CP002177 Genomic DNA. Translation: ADY81056.1.
RefSeqiWP_014206216.1. NC_016603.1.
YP_004994738.1. NC_016603.1.

3D structure databases

ProteinModelPortaliQ7WTJ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi871585.BDGL_000470.

Protein family/group databases

TCDBi5.B.1.8.2. the phagocyte (gp91(phox)) nadph oxidase family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADY81056; ADY81056; BDGL_000470.
GeneIDi11638648.
KEGGiacc:BDGL_000470.
PATRICi47078982. VBIAciCal168233_0467.

Phylogenomic databases

eggNOGiENOG4108JA0. Bacteria.
COG0543. LUCA.
KOiK16246.
OMAiYMTLERR.

Enzyme and pathway databases

UniPathwayiUPA00728.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR001221. Phe_hydroxylase.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00410. PHEHYDRXLASE.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDMPP_ACICP
AccessioniPrimary (citable) accession number: Q7WTJ2
Secondary accession number(s): F0KIB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 70 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.