Q7WTB4 (BGAL_LACHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase large subunit
Short name=Beta-gal large subunit
EC=3.2.1.23

Alternative name(s):
Lactase large subunit

Gene names

Name:

lacL

Organism

Lactobacillus helveticus (Lactobacillus suntoryeus)

Taxonomic identifier

1587 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subunit structure	Heterodimer of a large and a small subunit By similarity.
Induction	By lactose. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	Beta-galactosidase large subunit		PRO_0000057667
Sites
	Active site	468	1	Proton donor By similarity
	Active site	536	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WTB4 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: A4BBC9B78A84517A	FASTA	628	73,479
	10 20 30 40 50 60 MQANINWLDN PEVFRVNQLP AHSDHPFFRD YREWQKQHSS YQQSLNGKWK FHFSANPMDR 70 80 90 100 110 120 PQDFYQRDFD SSNFDSIPVP SEIELSNYTQ NQYINVLFPW EGKIFRRPAY ALDPNDHEEG 130 140 150 160 170 180 SFSKGADNTV GSYLKRFDLS SALIGKDVHI KFEGVEQAMY VWLNGHFVGY AEDSFTPSEF 190 200 210 220 230 240 DLTPYIQDKD NLLAVEVFKH STASWLEDQD MFRFSGIFRS VELLGIPATH LMDMDLKPRV 250 260 270 280 290 300 ADNYQDGIFN LKLHFIGKKA GSFHLLVKDI KGHTLLEKNE DIKENVQINN EKFENVHLWN 310 320 330 340 350 360 NHDPYLYQLL IEVYDEQQNL LELIPFQFGF RRIEISPEKV VLLNGKRLII NGVNRHEWDA 370 380 390 400 410 420 KRGRSITMSD MTTDINTFKE NNINAVRTCH YPNQIPWYYL CDQNGIYVMA ENNLESHGTW 430 440 450 460 470 480 QKMGEIEPSD NVPGSIPQWK EAVIDRARIN YETFKNHTSI LFWSLGNESY AGDNIIAMNE 490 500 510 520 530 540 FYKSHDDTRL VHYEGVVHRP ELKDKISDVE SCMYLPPKKV EEYLQNDPPK PFMECEYMHD 550 560 570 580 590 600 MGNSDGGMGS YIKLLDKYPQ YFGGFIWDFI DQALLVHDEI SGHDVLRYGG DFDDRHSDYE 610 620 FSGDGLMFAD RTPKPAMQEV RYYYGLHK

References

[1]

"Unusual organization for lactose and galactose gene clusters in Lactobacillus helveticus." Fortina M.G., Ricci G., Mora D., Guglielmetti S., Manachini P.L. Appl. Environ. Microbiol. 69:3238-3243(2003) [PubMed: 12788721] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TRANSCRIPTIONAL REGULATION. Strain: ATCC 15009 / DSM 20075 / JCM 1120 / NBRC 15019 / NCIMB 11971 / Lh12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ512877 Genomic DNA. Translation: CAD55499.1.
3D structure databases
ProteinModelPortal	Q7WTB4.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR023232. Glyco_hydro_2_AS. IPR023230. Glyco_hydro_2_CS. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_LACHE

Accession

Primary (citable) accession number: Q7WTB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2003
Last modified:	September 21, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program