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Protein

Beta-galactosidase large subunit

Gene

lacL

Organism
Lactobacillus helveticus (Lactobacillus suntoryeus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei468 – 4681Proton donorBy similarity
Active sitei536 – 5361NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase large subunit (EC:3.2.1.23)
Short name:
Beta-gal large subunit
Alternative name(s):
Lactase large subunit
Gene namesi
Name:lacL
OrganismiLactobacillus helveticus (Lactobacillus suntoryeus)
Taxonomic identifieri1587 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 628628Beta-galactosidase large subunitPRO_0000057667Add
BLAST

Expressioni

Inductioni

By lactose.1 Publication

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.By similarity

Protein-protein interaction databases

STRINGi405566.lhv_1549.

Structurei

3D structure databases

ProteinModelPortaliQ7WTB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7WTB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQANINWLDN PEVFRVNQLP AHSDHPFFRD YREWQKQHSS YQQSLNGKWK
60 70 80 90 100
FHFSANPMDR PQDFYQRDFD SSNFDSIPVP SEIELSNYTQ NQYINVLFPW
110 120 130 140 150
EGKIFRRPAY ALDPNDHEEG SFSKGADNTV GSYLKRFDLS SALIGKDVHI
160 170 180 190 200
KFEGVEQAMY VWLNGHFVGY AEDSFTPSEF DLTPYIQDKD NLLAVEVFKH
210 220 230 240 250
STASWLEDQD MFRFSGIFRS VELLGIPATH LMDMDLKPRV ADNYQDGIFN
260 270 280 290 300
LKLHFIGKKA GSFHLLVKDI KGHTLLEKNE DIKENVQINN EKFENVHLWN
310 320 330 340 350
NHDPYLYQLL IEVYDEQQNL LELIPFQFGF RRIEISPEKV VLLNGKRLII
360 370 380 390 400
NGVNRHEWDA KRGRSITMSD MTTDINTFKE NNINAVRTCH YPNQIPWYYL
410 420 430 440 450
CDQNGIYVMA ENNLESHGTW QKMGEIEPSD NVPGSIPQWK EAVIDRARIN
460 470 480 490 500
YETFKNHTSI LFWSLGNESY AGDNIIAMNE FYKSHDDTRL VHYEGVVHRP
510 520 530 540 550
ELKDKISDVE SCMYLPPKKV EEYLQNDPPK PFMECEYMHD MGNSDGGMGS
560 570 580 590 600
YIKLLDKYPQ YFGGFIWDFI DQALLVHDEI SGHDVLRYGG DFDDRHSDYE
610 620
FSGDGLMFAD RTPKPAMQEV RYYYGLHK
Length:628
Mass (Da):73,479
Last modified:October 1, 2003 - v1
Checksum:iA4BBC9B78A84517A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ512877 Genomic DNA. Translation: CAD55499.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ512877 Genomic DNA. Translation: CAD55499.1.

3D structure databases

ProteinModelPortaliQ7WTB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405566.lhv_1549.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Unusual organization for lactose and galactose gene clusters in Lactobacillus helveticus."
    Fortina M.G., Ricci G., Mora D., Guglielmetti S., Manachini P.L.
    Appl. Environ. Microbiol. 69:3238-3243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TRANSCRIPTIONAL REGULATION.
    Strain: ATCC 15009 / DSM 20075 / JCM 1120 / NBRC 15019 / NCIMB 11971 / Lh12.

Entry informationi

Entry nameiBGAL_LACHE
AccessioniPrimary (citable) accession number: Q7WTB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 1, 2003
Last modified: June 24, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.