Reviewed, UniProtKB/Swiss-Prot Q7WR85 (AROK_BORBR)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	BB0071

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00109
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity. HAMAP MF_00109
Subcellular location	Cytoplasm Probable HAMAP MF_00109.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Shikimate kinase HAMAP MF_00109		PRO_0000237850
Regions
	Nucleotide binding	50 – 55	6	ATP By similarity
Sites
	Metal binding	54	1	Magnesium By similarity
	Binding site	72	1	Substrate By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	118	1	Substrate; via amide nitrogen By similarity
	Binding site	156	1	ATP By similarity
	Binding site	175	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WR85-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: EB8502512BFBD5EA	FASTA	211	23,083
	10 20 30 40 50 60 MNASANLCAA SDNDPQPGDQ EAAHPVACAG DEPAAFLPHD LPIFLVGMMG AGKTTIGRGL 70 80 90 100 110 120 ARALRREFID LDHELEARCG VRVPVIFEIE GEAGFRRREA AALQECTQRR QIILATGGGA 130 140 150 160 170 180 VLAAENRQAL RERGIVIYLR ASVEELFRRT SRDRNRPLLA TADPRATLRE LMVAREPLYN 190 200 210 EVADLVIDTG SMPIATLVKS LLPKLQAYEK K

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640437 Genomic DNA. Translation: CAE30573.1.
RefSeq	NP_886624.1.
3D structure databases
SMR	Q7WR85. Positions 43-205.
ModBase	Search...
Genome annotation databases
GeneID	2662361.
KEGG	bbr:BB0071.
NMPDR	fig|257310.1.peg.71.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335815.
OMA	HANVFFV.
PhylomeDB	Q7WR85.
Enzyme and pathway databases
BioCyc	BBRO257310:BB0071-MONOMER.
BRENDA	2.7.1.71. 413.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_BORBR

Accession

Primary (citable) accession number: Q7WR85

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)