Reviewed, UniProtKB/Swiss-Prot Q7WR61 (MASZ_BORBR)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	BB0095

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Malate synthase G HAMAP MF_00641		PRO_1000056893
Sites
	Active site	339	1	Proton acceptor By similarity
	Active site	632	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WR61-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 56CFA5BAAD16127C	FASTA	725	78,547
	10 20 30 40 50 60 MTERIPHHGL QVAASLHRFI EDEALSGSGL APDEFWAGFA ALVRDLAPRN RELLAERDRL 70 80 90 100 110 120 QGEIDAWHRA HPGPVRDSAG YQALLERIGY LQPQPAQVTA STRDVDSEIA SQAGPQLVVP 130 140 150 160 170 180 VSNARYALNA ANARWGSLYD ALYGTDAIPP VAGDDGKGYN PARGEAVIAR ARAFLDEAAP 190 200 210 220 230 240 LAQGSHADAT AYAIEGGKLA VTLGAGQRTG LRNPAQLAGY QGDASQPAAV LLANNGLHFE 250 260 270 280 290 300 IQIDRQHQIG ATDAAGVKDV LLEAALTTIM DCEDSVAAVD ADDKVLIYRN WLGLMKGDLS 310 320 330 340 350 360 ESVTKGGKTF TRRLNADRQY HKPDGGTLTL HGRSLMFVRN VGHLMTNPAI LDEQGNEVPE 370 380 390 400 410 420 GILDAVITSL AALPDRANRL NSRTGSIYIV KPKMHGPAEA AFANELFDRV EDLLKLPRHT 430 440 450 460 470 480 IKMGIMDEER RTSVNLKACI AAAAARVAFI NTGFLDRTGD EMHTGMEAGP MLRKGDMKSS 490 500 510 520 530 540 AWITAYERNN VLVGLDCGLR GRAQIGKGMW AMPDMMAAML EQKIGHPKAG ANTAWVPSPT 550 560 570 580 590 600 AATLHAMHYH QVDVAAVQQA LEQTRYDSVR DELLAGLLTV PVGDPAAWSA DDIQRELDNN 610 620 630 640 650 660 AQGILGYVVR WIDQGVGCSK VPDINNVGLM EDRATLRISS QHIANWLRHG IVDRAQVNAT 670 680 690 700 710 720 FERMAKVVDQ QNAGDPNYLP MAGHFDTSFA YRAACALVFE GLTQPNGYTE PLLHEYRQAF KAAQR

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640437 Genomic DNA. Translation: CAE30597.1.
RefSeq	NP_886648.1.
3D structure databases
HSSP	HSSP built from PDB template 1P7T based on UniProtKB P37330.
ModBase	Search...
Genome annotation databases
GeneID	2662908.
GenomeReviews	Gene locus BB0095 in contig BX470250_GR.
KEGG	bbr:BB0095.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7WR61.
OMA	SQFIENE.
Enzyme and pathway databases
BioCyc	BBRO257310:BB0095-MON.
BRENDA	2.3.3.9. 413.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_BORBR

Accession

Primary (citable) accession number: Q7WR61

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)