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Q7WP45 (SYM_BORBR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:BB0840
OrganismBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]
Taxonomic identifier257310 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length692 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 692692Methionine--tRNA ligase HAMAP MF_00098
PRO_0000139106

Regions

Domain586 – 692107tRNA-binding
Motif12 – 2211"HIGH" region HAMAP MF_00098
Motif341 – 3455"KMSKS" region HAMAP MF_00098

Sites

Metal binding1431Zinc By similarity
Metal binding1461Zinc By similarity
Metal binding1561Zinc By similarity
Metal binding1591Zinc By similarity
Binding site3441ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7WP45 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: F49E00DD2F53444A

FASTA69276,486
        10         20         30         40         50         60 
MSRTLFVTTA LPYANGSFHI GHIMEYIQAD IWVRSMRMAG HTVHFVGADD AHGAPIMLKA 

        70         80         90        100        110        120 
EKEGITPQAL VARYAAERPR YLDGFHIRFD HWHSTDTPEN VALSQEIYRA LKSEGLIETR 

       130        140        150        160        170        180 
SIEQFYDPVK GMFLADRYIK GECPRCHAKD QYGDSCEVCG AVYAPTELIN PYSALTGAAP 

       190        200        210        220        230        240 
VLKSSDHFFF KLSDPRCVEF LQQWTTGANR QGVKHLQAEV QAKTREWLGG DDGEAKLGDW 

       250        260        270        280        290        300 
DISRDAPYFG IEIPDAPGKY FYVWLDAPVG YLASLKSYCA VKGLDFDALL DPAGPTEQVH 

       310        320        330        340        350        360 
FIGKDIIYFH ALFWPAMLKF AGRKTPDQLN VHGFITVSGE KMSKSRGTGI SPLRYLEIGM 

       370        380        390        400        410        420 
DAEWLRYYMA AKLNARVEDM DFNPEDFVAR VNSDLVGKYV NIASRAAAFI TRHFDGELAY 

       430        440        450        460        470        480 
DGDTDALAAE FAQQAESIRA AFEAREYNRA VREIMAHADR INQAFDAAQP WVMAKGIGAA 

       490        500        510        520        530        540 
DAATRARLQD ICSRALAGFK ALSVMLAPVL PALASRVARE LFGANADFAW GDAQQLPQRV 

       550        560        570        580        590        600 
APFKHLMQRV DPKLLDDLFE PPAAEASAPA ALPGGEALAD TITIDDFAKI DLRIARIVNC 

       610        620        630        640        650        660 
EEVEGSTKLL RLTLDVGEGR HRNVFSGIKS AYQPQDLVGK LTVLVANLAP RKMKFGVSEG 

       670        680        690 
MVLAASHADE KAEPGIYVLE PWPGAQPGMR VR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX640439 Genomic DNA. Translation: CAE31339.1.
RefSeqNP_887389.1. NC_002927.3.

3D structure databases

ProteinModelPortalQ7WP45.
SMRQ7WP45. Positions 135-161.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2662481.
GenomeReviewsGene locus BB0840 in contig BX470250_GR.
KEGGbbr:BB0840.
NMPDRfig|257310.1.peg.836.
PATRIC21134897. VBIBorBro124907_0846.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG721667.
OMAEEKTRNV.
PhylomeDBQ7WP45.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycBBRO257310:BB0840-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BORBR
AccessionPrimary (citable) accession number: Q7WP45
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 1, 2003
Last modified: January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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