Reviewed, UniProtKB/Swiss-Prot Q7WP45 (SYM_BORBR)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BB0840

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Homodimer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 692	692	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000139106
Regions
	Domain	586 – 692	107	tRNA-binding
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	341 – 345	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	344	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WP45-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: F49E00DD2F53444A	FASTA	692	76,486
	10 20 30 40 50 60 MSRTLFVTTA LPYANGSFHI GHIMEYIQAD IWVRSMRMAG HTVHFVGADD AHGAPIMLKA 70 80 90 100 110 120 EKEGITPQAL VARYAAERPR YLDGFHIRFD HWHSTDTPEN VALSQEIYRA LKSEGLIETR 130 140 150 160 170 180 SIEQFYDPVK GMFLADRYIK GECPRCHAKD QYGDSCEVCG AVYAPTELIN PYSALTGAAP 190 200 210 220 230 240 VLKSSDHFFF KLSDPRCVEF LQQWTTGANR QGVKHLQAEV QAKTREWLGG DDGEAKLGDW 250 260 270 280 290 300 DISRDAPYFG IEIPDAPGKY FYVWLDAPVG YLASLKSYCA VKGLDFDALL DPAGPTEQVH 310 320 330 340 350 360 FIGKDIIYFH ALFWPAMLKF AGRKTPDQLN VHGFITVSGE KMSKSRGTGI SPLRYLEIGM 370 380 390 400 410 420 DAEWLRYYMA AKLNARVEDM DFNPEDFVAR VNSDLVGKYV NIASRAAAFI TRHFDGELAY 430 440 450 460 470 480 DGDTDALAAE FAQQAESIRA AFEAREYNRA VREIMAHADR INQAFDAAQP WVMAKGIGAA 490 500 510 520 530 540 DAATRARLQD ICSRALAGFK ALSVMLAPVL PALASRVARE LFGANADFAW GDAQQLPQRV 550 560 570 580 590 600 APFKHLMQRV DPKLLDDLFE PPAAEASAPA ALPGGEALAD TITIDDFAKI DLRIARIVNC 610 620 630 640 650 660 EEVEGSTKLL RLTLDVGEGR HRNVFSGIKS AYQPQDLVGK LTVLVANLAP RKMKFGVSEG 670 680 690 MVLAASHADE KAEPGIYVLE PWPGAQPGMR VR

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640439 Genomic DNA. Translation: CAE31339.1.
RefSeq	NP_887389.1.
3D structure databases
SMR	Q7WP45. Positions 2-560, 581-691.
ModBase	Search...
Genome annotation databases
GeneID	2662481.
KEGG	bbr:BB0840.
NMPDR	fig|257310.1.peg.836.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG721667.
OMA	SAYQPEQ.
PhylomeDB	Q7WP45.
Enzyme and pathway databases
BioCyc	BBRO257310:BB0840-MONOMER.
BRENDA	6.1.1.10. 413.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BORBR

Accession

Primary (citable) accession number: Q7WP45

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)