Reviewed, UniProtKB/Swiss-Prot Q7WP13 (TYSY_BORBR)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BB0872

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Thymidylate synthase HAMAP MF_00008		PRO_0000140938
Sites
	Active site	192	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WP13-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 39E014AE19B3C0AC	FASTA	323	36,406
	10 20 30 40 50 60 MKQYLDLVQS ILDQGAWQEN RTGVRTLSLP GAALRFDLQQ GFPAVTTKKL AFKSAIGEMV 70 80 90 100 110 120 GFLRATRSAA QFRALGCKVW DQNANENEQW LANPYREGPD DLGPVYGVQW RHWPAYKLLP 130 140 150 160 170 180 RSAGGQVADA LARGYRQVAE VAENGAPHVL LYKAVDQLRQ CLDTIHQSPG DRRILFHGWN 190 200 210 220 230 240 WAQIEEMALP PCHLLYQFLP NAGTREISLC LYIRSNDVGL GTPFNLTEGA ALLHLVGRLT 250 260 270 280 290 300 GYKPRWFSYF IGDAHVYENH LPMLREQLTR EPYPAPQLVL SDRIPDFAVT GKYEPQWLEQ 310 320 VEPGDFTLSG YQHHAPLTAP MAV

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640439 Genomic DNA. Translation: CAE31371.1.
RefSeq	NP_887421.1.
3D structure databases
HSSP	HSSP built from PDB template 1HW3 based on UniProtKB P04818.
ModBase	Search...
Genome annotation databases
GeneID	2660120.
GenomeReviews	Gene locus BB0872 in contig BX470250_GR.
KEGG	bbr:BB0872.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7WP13.
OMA	LGPVYGH.
Enzyme and pathway databases
BioCyc	BBRO257310:BB0872-MON.
BRENDA	2.1.1.45. 413.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BORBR

Accession

Primary (citable) accession number: Q7WP13

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)