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Q7WNY5 (ISPE_BORBR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name:ispE
Synonyms:ipk
Ordered Locus Names:BB0900
OrganismBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]
Taxonomic identifier257310 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
   Biological processIsoprene biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processterpenoid biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular function4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity

Inferred from electronic annotation. Source: EC

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2992994-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
PRO_0000189191

Regions

Nucleotide binding94 – 10411ATP Potential

Sites

Active site111 By similarity
Active site1361 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7WNY5 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 8B01D623620C0A91

FASTA29931,897
        10         20         30         40         50         60 
MTLYDVPAPA KLNLFLHVVG RRADGYHLLQ TAFRFIDLAD TLHFEARADG AIGRAYELPG 

        70         80         90        100        110        120 
VAESDDLVVR AARSLQRATG TRQGAQIGLH KRIPQGGGLG GGSSDAATTL IALNRLWGTG 

       130        140        150        160        170        180 
LSRSQLMQLA LPLGADVPVF VFGQSAFAQG VGEDLTAVAL PPAAYLVVQP DAGVPTAAIF 

       190        200        210        220        230        240 
SDPDLTRDCA SVTIADFLAL PTFCFGRNDL EPVVLRRYPE VSGAVRWLFE HGLRVRMSGS 

       250        260        270        280        290 
GACLFAEFPT LPEAVLAQEE ITATMRVAGK TTSHTHPGFR LVQASTGLTE HPLRNWIAS

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX640439 Genomic DNA. Translation: CAE31399.1.
RefSeqNP_887449.1. NC_002927.3.

3D structure databases

ProteinModelPortalQ7WNY5.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2663204.
GenomeReviewsGene locus BB0900 in contig BX470250_GR.
KEGGbbr:BB0900.
NMPDRfig|257310.1.peg.896.
PATRIC21135025. VBIBorBro124907_0910.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG734593.
OMALNLFLHV.
PhylomeDBQ7WNY5.
ProtClustDBPRK00343.

Enzyme and pathway databases

BioCycBBRO257310:BB0900-MONOMER.

Family and domain databases

HAMAPMF_00061. IspE.
[Tree]
InterProIPR006204. GHMP_kinase.
IPR013750. GHMP_kinase_C.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
Gene3DG3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KOK00919.
PANTHERPTHR20861:SF2. IspE. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
SUPFAMSSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMsTIGR00154. IspE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_BORBR
AccessionPrimary (citable) accession number: Q7WNY5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 1, 2003
Last modified: January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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