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Q7WNM3 (LEU31_BORBR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydrogenase 1
EC=1.1.1.85
Alternative name(s):
3-IPM-DH 1
Beta-IPM dehydrogenase 1
Short name=IMDH 1
Gene names
Name:leuB1
Ordered Locus Names:BB1014
OrganismBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]
Taxonomic identifier257310 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity. HAMAP MF_01033

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033

Subunit structure

Homodimer By similarity. HAMAP MF_01033

Subcellular location

Cytoplasm By similarity HAMAP MF_01033.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3703703-isopropylmalate dehydrogenase 1 HAMAP MF_01033
PRO_0000250103

Regions

Nucleotide binding289 – 30113NAD By similarity

Sites

Metal binding2271Magnesium or manganese By similarity
Metal binding2511Magnesium or manganese By similarity
Metal binding2551Magnesium or manganese By similarity
Binding site981Substrate By similarity
Binding site1081Substrate By similarity
Binding site1361Substrate By similarity
Binding site2271Substrate By similarity
Site1431Important for catalysis By similarity
Site1941Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7WNM3 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: E58A5488FBF1034F

FASTA37039,907
        10         20         30         40         50         60 
MSEQNRLLLI GGDGVGAEVV HEAGRIAQWF AARRGLPLSI GQAAFGVEVL NRTGRIMTDD 

        70         80         90        100        110        120 
TLAQIRAADA VLFGAIGGPE YDRLPLEQVR EEGLLRIRRE LGLYANLRPV RYWPALAELC 

       130        140        150        160        170        180 
PFVPERVEKV DMMIVRELAG GIYYASPRGI DQTGDGRRVG TNTQRYDDLE IRRIARVAFE 

       190        200        210        220        230        240 
LARARGGRVC SVDKSNVLES GLLWREEVQA CRDAEYPDVE LEHILVDNCA LQLCLRPARF 

       250        260        270        280        290        300 
DVLLADNLFG DILSDAAGAV AGSLGMLPSA SFGAEDGGRR RGFYEPVHGS APDIAGQGIA 

       310        320        330        340        350        360 
NPLGAILSLA LALRWTFRQP AEADLLERAV ERALAGGART LDLLAGAAAP ALSTRAMADA 

       370 
VLQALETLAA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX640440 Genomic DNA. Translation: CAE31513.1.
RefSeqNP_887562.1. NC_002927.3.

3D structure databases

HSSPHSSP built from PDB template 1A05 based on UniProtKB Q56268.
ProteinModelPortalQ7WNM3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2663175.
GenomeReviewsGene locus BB1014 in contig BX470250_GR.
KEGGbbr:BB1014.
NMPDRfig|257310.1.peg.1009.
PATRIC21135262. VBIBorBro124907_1027.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG518924.
PhylomeDBQ7WNM3.

Enzyme and pathway databases

BioCycBBRO257310:BB1014-MONOMER.

Family and domain databases

HAMAPMF_01033. LeuB_type1.
[Tree]
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KOK00052.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. LeuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU31_BORBR
AccessionPrimary (citable) accession number: Q7WNM3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: October 1, 2003
Last modified: January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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