Reviewed, UniProtKB/Swiss-Prot Q7WKM7 (METE_BORBR)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BB2079

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098615
Sites
	Metal binding	652	1	Zinc By similarity
	Metal binding	654	1	Zinc By similarity
	Metal binding	737	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WKM7-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: FBF3379815D909EF	FASTA	764	84,332
	10 20 30 40 50 60 MTIIHNLGFP RIGAQRELKR AVEAYWAGRQ TAEALHETGR ALRAAHWQRQ ADAGVAFVPV 70 80 90 100 110 120 GDFAWYDHIL EWTTLLGAVP ARFGHPEGKP VDLDTLFRMG RGRAPSGKPA AACEMTKWFD 130 140 150 160 170 180 TNYHYIVPEL TPGQTFRVAR EDLFEQVKEA QALGHRVKPV IPGPLTWLWL GKGDAFAQGA 190 200 210 220 230 240 GDIGKLQLLD ALLPVYGEVL ERLAGLGVEW VQIDEPALVL DLPQAWRDAY QAVYAKLAAS 250 260 270 280 290 300 PVKLLLATYF DGLKDNLATA LALPVAGLHV DLVRAPDQLS DVASGLRPGQ VLSAGVINGR 310 320 330 340 350 360 NIWRTDLDAA LAMLAPVREQ LQERLWLAPS CSLLHVPVDL AGETELDAEL LGWLSFAVQK 370 380 390 400 410 420 LDELCLLGKA LGGDADPAVQ QGLAAQRAAL QARRQSPRIH NPAVAQRMAG QAGVSRERAP 430 440 450 460 470 480 FGQRIARQQS ELRLPAFPTT TIGSFPQTAE IRALRRDWKS GALTDSAYEN AIRKEIEEVI 490 500 510 520 530 540 RFQEKVGLDV LVHGEPERND MVEYFGELLA GFAFTKNGWV QSYGSRCVKP PIIFGDVARP 550 560 570 580 590 600 APMTVGWSAY AQSLTDKPVK GMLTGPVTIL QWSFVRDDQP REATCRQLAL ALRDEVVDLE 610 620 630 640 650 660 AAGIRVIQID EPAIREGLPL RRADWRAYLD WAVDCFRLST AGVGEATQIH THMCYSEFND 670 680 690 700 710 720 IIESIAAMDA DVITIETSRS NMELLKAFED FHYPNDIGPG VYDIHSPNVP EVDWMVELMR 730 740 750 760 KAAARLPKER LWVNPDCGLK TRAWPETEAA LIGMVQAART LRAA

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640443 Genomic DNA. Translation: CAE32575.1.
RefSeq	NP_888622.1.
3D structure databases
SMR	Q7WKM7. Positions 4-763.
ModBase	Search...
Genome annotation databases
GeneID	2660297.
KEGG	bbr:BB2079.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	SFVRNDI.
PhylomeDB	Q7WKM7.
Enzyme and pathway databases
BioCyc	BBRO257310:BB2079-MONOMER.
BRENDA	2.1.1.14. 413.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BORBR

Accession

Primary (citable) accession number: Q7WKM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)