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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateUniRule annotation
Binding sitei226 – 2261Substrate; via amide nitrogenUniRule annotation
Metal bindingi233 – 2331ManganeseUniRule annotation
Binding sitei233 – 2331SubstrateUniRule annotation
Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
Binding sitei275 – 2751SubstrateUniRule annotation
Active sitei277 – 2771UniRule annotation
Metal bindingi302 – 3021ManganeseUniRule annotation
Binding sitei398 – 3981GTPUniRule annotation
Binding sitei429 – 4291GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816GTPUniRule annotation
Nucleotide bindingi526 – 5294GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBBRO257310:BB2434-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pck
Ordered Locus Names:BB2434
OrganismiBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Taxonomic identifieri257310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
ProteomesiUP000001027: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103590Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi257310.BB2434.

Structurei

3D structure databases

ProteinModelPortaliQ7WJQ9.
SMRiQ7WJQ9. Positions 17-617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni396 – 3983Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7WJQ9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKPSEVKPV ALNVPDYVKH SGLIAWVERI AALTKPDRVV WCDGSQEEYD
60 70 80 90 100
RLCEQMVQGG TMRRLNPAKR ANSYLACSDP SDVARVEDRT FICSEREEDA
110 120 130 140 150
GPTNNWAAPQ AMRETLGGLF DGAMRGRTMY VVPFSMGPLG SPIAHIGVEL
160 170 180 190 200
SDSPYVVVNM RIMTRMGRKV FDVLGSDGAF VPCVHSVGMP LAEGQQDVAW
210 220 230 240 250
PCNPTKYIVH YPETREIWSF GSGYGGNALL GKKCFALRIA STMGRDEGWL
260 270 280 290 300
AEHMLILGVT SPKGRKYHVA AAFPSACGKT NFAMLIPPHG MDGWKVTTIG
310 320 330 340 350
DDIAWIKPGP DGRLHAINPE AGYFGVAPGT SEKTNFNAMA TLKANVIFTN
360 370 380 390 400
VALTDDGDVW WEGMTDTPPA HLIDWQGQDW TPAIAAETGR KAAHPNARFT
410 420 430 440 450
APAAQCPSID PEWENPKGVA IDAFIFGGRR STTVPLVTEA RNWVEGVYMA
460 470 480 490 500
ATMGSETTAA AAGQQGVVRR DPFAMLPFCG YNMSDYFDHW LKLGRRLEET
510 520 530 540 550
GATLPRIYCV NWFRKGPDGK FVWPGFGENM RVLRWMLGRL DGQAGGVDQV
560 570 580 590 600
FGISPAYGDI DWTGLEFSPD KFEQVISVDL PAWRAELALH DELFTQLAAR
610
LPEDLPLTRT AIERRMAA
Length:618
Mass (Da):67,954
Last modified:October 1, 2003 - v1
Checksum:i5309E81920252DAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640444 Genomic DNA. Translation: CAE32928.1.
RefSeqiNP_888974.1. NC_002927.3.

Genome annotation databases

EnsemblBacteriaiCAE32928; CAE32928; BB2434.
GeneIDi2659771.
KEGGibbr:BB2434.
PATRICi21138195. VBIBorBro124907_2467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640444 Genomic DNA. Translation: CAE32928.1.
RefSeqiNP_888974.1. NC_002927.3.

3D structure databases

ProteinModelPortaliQ7WJQ9.
SMRiQ7WJQ9. Positions 17-617.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257310.BB2434.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE32928; CAE32928; BB2434.
GeneIDi2659771.
KEGGibbr:BB2434.
PATRICi21138195. VBIBorBro124907_2467.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciBBRO257310:BB2434-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica."
    Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S.
    , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J.
    Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-588 / NCTC 13252 / RB50.

Entry informationi

Entry nameiPCKG_BORBR
AccessioniPrimary (citable) accession number: Q7WJQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: October 1, 2003
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.