Reviewed, UniProtKB/Swiss-Prot Q7WJP7 (ILVD2_BORBR)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 2
      Short name=DAD 2
    EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	BB2446

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000103435
Sites
	Metal binding	124	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	196	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7WJP7-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 810FE9D4B26A4FF0	FASTA	570	58,917
	10 20 30 40 50 60 MSLHKHRSRM VTQGLTRTPH RAFLRATGFD DAALEKSMVG IVSTQGENTP CSMALGPQAD 70 80 90 100 110 120 RARLGVAAGG GVPVSFSTIS ISDGTSMNHA GMRMSLLSRE TIADSVELVV RGHAYDALVA 130 140 150 160 170 180 FAGCDKTLPA MMMAIVRLNV PAVFLYGGAT LPGHAASGQV TILDTIEAVG RVQHGTMPAA 190 200 210 220 230 240 ELKAMERTCT PSAGSCPGQF TANTMAMVGE ALGLSPLGSA MMPAVYSERL AIAQRAGEHV 250 260 270 280 290 300 MRALAAGGPL PRQLVTRASL ENACAAVAAT GGSTNAALHI PAIAHEAGIA FTLDDVARVF 310 320 330 340 350 360 KRTPLIADLQ PGGRYLARDL HEAGGVPVVL KALLDGGHID GSVLTLDGRT LAEALADTPA 370 380 390 400 410 420 PDGKVVRACG QALHPTGGVA VLKGNLSPDG ALLKIAGLKS LKFSGPARVF ENEEACMRAV 430 440 450 460 470 480 STHAYQPGEV LVIRNEGPRG GPGMREMLSV TAALYGQGMG EKVALLTDGR FSGATRGLCI 490 500 510 520 530 540 GYAGPEAAAG GPIALLRDGD MIHIDAEADT LDVALSDEEL ARRRAAYQAP PQARLAGALE 550 560 570 KYAALVRPAC LGAVTHSGQV DWPYETPEAE

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640444 Genomic DNA. Translation: CAE32940.1.
RefSeq	NP_888986.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2662097.
KEGG	bbr:BB2446.
NMPDR	fig|257310.1.peg.2433.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	RGLCIGY.
PhylomeDB	Q7WJP7.
Enzyme and pathway databases
BioCyc	BBRO257310:BB2446-MONOMER.
BRENDA	4.2.1.9. 413.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_BORBR

Accession

Primary (citable) accession number: Q7WJP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)