Q7WJ84 (LPXD_BORBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	BB2615

Organism

Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

257310 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000059648
Sites
	Active site	266	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WJ84 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 7A5C4C8EB4305445	FASTA	363	37,528
	10 20 30 40 50 60 MPVLLDPENA LALDVLLAGT DAQGLDWHLS APDAADLPRI RGIGTLSSAG SEEISFLSNP 70 80 90 100 110 120 RYQNQLATTR AAAVIVTPDV AQARQEQGAS GHVLVVCKHP YLLYARLAQW FERASRPAGP 130 140 150 160 170 180 AGVHPSAVVD PSAEIDADAR VGAQCVIEAG ARIGRGARLG PGCVIGAGST VGADSLLHPR 190 200 210 220 230 240 VTLYAGVHVG ERAIIHSGAV LGADGFGFAP DPTLGRGAWG KIPQLGGVRV GNDVEIGANT 250 260 270 280 290 300 TIDRGALDDT IVGDGVKLDN QIMVAHNVRI GAHTAIAACV GIAGSTTIGE RCTIGGASML 310 320 330 340 350 360 SGHLAIADDV NISGGTAVTS NIAKAGRYTG VYPYAEHSEW QRNAAVIQQL ALLRRRLRAL ERE

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-588 / NCTC 13252 / RB50.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640444 Genomic DNA. Translation: CAE33108.1.
RefSeq	NP_889152.1. NC_002927.3.
3D structure databases
ProteinModelPortal	Q7WJ84.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2661817.
GenomeReviews	Gene locus BB2615 in contig BX470250_GR.
KEGG	bbr:BB2615.
NMPDR	fig|257310.1.peg.2599.
PATRIC	21138565. VBIBorBro124907_2651.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	Q7WJ84.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	BBRO257310:BB2615-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 3 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_BORBR

Accession

Primary (citable) accession number: Q7WJ84

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program