Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q7WIQ1

- NAPA_BORBR

UniProt

Q7WIQ1 - NAPA_BORBR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBBRO257310:BB2800-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:BB2800
OrganismiBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Taxonomic identifieri257310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
ProteomesiUP000001027: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3636Tat-type signalUniRule annotationAdd
BLAST
Chaini37 – 829793Periplasmic nitrate reductasePRO_0000045977Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi257310.BB2800.

Structurei

3D structure databases

ProteinModelPortaliQ7WIQ1.
SMRiQ7WIQ1. Positions 39-826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7WIQ1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARRDFIKQT AAAAAATVAG VPLTGYTQNI VTESEAAKLK WSKAPCRFCG
60 70 80 90 100
TGCGVNVAVK DNQVVATHGD FNAEVNKGLN CVKGYFLSKI MYGSDRLTQP
110 120 130 140 150
LLRMKDGKYA KDGEFAPVSW DQAFDVMAEQ FKRVLKDKGP EAVGMFGSGQ
160 170 180 190 200
WTVWEGYAAL KLMKAGFRTN NLDPNARHCM ASAAVGFMRT FGADEPMGCY
210 220 230 240 250
DDIENADAFV LWGSNMAEMH PILWTRVTDR RLSAPATKVA VLSTFEHRSY
260 270 280 290 300
ELADLTLTFE PQSDLAILNY IANHIIRTKR VNRDFVDKHT VFREGNADIG
310 320 330 340 350
YGLRPEHPLQ QAARNAGDAG GSKPITFDDF ARFVSKYDLE YTAKLSGVPK
360 370 380 390 400
NRLEELAELY ADPKVRVTSF WTMGFNQHTR GVWCNNMVYN IHLLTGKIST
410 420 430 440 450
PGNSPFSLTG QPSACGTARE VGTFSHRLPA DLVVTNPEHR RHAEEIWKLP
460 470 480 490 500
DGTIPSKVGA HAVLQNRMLK DGKINAYWVM VNNNMQAAAN LMNEGLPGYR
510 520 530 540 550
NPENFIVVSD AYPTVTTLSA DLILPAAMWV EKEGAYGNAE RRTQFWHQLV
560 570 580 590 600
DAPGQARSDL WQLVEFSKRF KVEEVWPADL LAKKPEYRGK TLYDVLFANG
610 620 630 640 650
KVNQFPNTEL DAEYANQEAQ AFGFYLQKGL FEEYAEFGRG HGHDLAPFDV
660 670 680 690 700
YHKARGLRWP VVDGKETLWR YREGSDPYVK PGTGFQFYGN PDGKAVIFAL
710 720 730 740 750
PYEPPPEAPD KEYPFWLSTG RVLEHWHSGS MTRRVPELYK AFPEAVCFMH
760 770 780 790 800
PDDAQALGVR RGVEVEVVSR RGRMRTRVET RGRDKPPRGL VFVPWFDAGQ
810 820
LINKVTLDAT DPISFQTDFK KCAVKIVKV
Length:829
Mass (Da):92,970
Last modified:October 1, 2003 - v1
Checksum:i7E55710BACD97B3A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX640445 Genomic DNA. Translation: CAE33292.1.
RefSeqiNP_889336.1. NC_002927.3.
WP_010926645.1. NC_002927.3.

Genome annotation databases

EnsemblBacteriaiCAE33292; CAE33292; BB2800.
GeneIDi2661370.
KEGGibbr:BB2800.
PATRICi21138941. VBIBorBro124907_2839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX640445 Genomic DNA. Translation: CAE33292.1 .
RefSeqi NP_889336.1. NC_002927.3.
WP_010926645.1. NC_002927.3.

3D structure databases

ProteinModelPortali Q7WIQ1.
SMRi Q7WIQ1. Positions 39-826.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257310.BB2800.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE33292 ; CAE33292 ; BB2800 .
GeneIDi 2661370.
KEGGi bbr:BB2800.
PATRICi 21138941. VBIBorBro124907_2839.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci BBRO257310:BB2800-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica."
    Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S.
    , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J.
    Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-588 / NCTC 13252 / RB50.

Entry informationi

Entry nameiNAPA_BORBR
AccessioniPrimary (citable) accession number: Q7WIQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2003
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3