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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi46Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi53Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi81Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei83Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei150Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei175Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei179Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei373Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei377Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei483Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei532Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei559Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei795Substrate; via amide nitrogenUniRule annotation1
Binding sitei803Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei820Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:BB2800
OrganismiBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Taxonomic identifieri257310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000001027 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Tat-type signalUniRule annotationAdd BLAST36
ChainiPRO_000004597737 – 829Periplasmic nitrate reductaseUniRule annotationAdd BLAST793

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi257310.BB2800

Structurei

3D structure databases

ProteinModelPortaliQ7WIQ1
SMRiQ7WIQ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 954Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 219Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni243 – 247Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni262 – 264Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni509 – 510Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni719 – 728Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7WIQ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRDFIKQT AAAAAATVAG VPLTGYTQNI VTESEAAKLK WSKAPCRFCG
60 70 80 90 100
TGCGVNVAVK DNQVVATHGD FNAEVNKGLN CVKGYFLSKI MYGSDRLTQP
110 120 130 140 150
LLRMKDGKYA KDGEFAPVSW DQAFDVMAEQ FKRVLKDKGP EAVGMFGSGQ
160 170 180 190 200
WTVWEGYAAL KLMKAGFRTN NLDPNARHCM ASAAVGFMRT FGADEPMGCY
210 220 230 240 250
DDIENADAFV LWGSNMAEMH PILWTRVTDR RLSAPATKVA VLSTFEHRSY
260 270 280 290 300
ELADLTLTFE PQSDLAILNY IANHIIRTKR VNRDFVDKHT VFREGNADIG
310 320 330 340 350
YGLRPEHPLQ QAARNAGDAG GSKPITFDDF ARFVSKYDLE YTAKLSGVPK
360 370 380 390 400
NRLEELAELY ADPKVRVTSF WTMGFNQHTR GVWCNNMVYN IHLLTGKIST
410 420 430 440 450
PGNSPFSLTG QPSACGTARE VGTFSHRLPA DLVVTNPEHR RHAEEIWKLP
460 470 480 490 500
DGTIPSKVGA HAVLQNRMLK DGKINAYWVM VNNNMQAAAN LMNEGLPGYR
510 520 530 540 550
NPENFIVVSD AYPTVTTLSA DLILPAAMWV EKEGAYGNAE RRTQFWHQLV
560 570 580 590 600
DAPGQARSDL WQLVEFSKRF KVEEVWPADL LAKKPEYRGK TLYDVLFANG
610 620 630 640 650
KVNQFPNTEL DAEYANQEAQ AFGFYLQKGL FEEYAEFGRG HGHDLAPFDV
660 670 680 690 700
YHKARGLRWP VVDGKETLWR YREGSDPYVK PGTGFQFYGN PDGKAVIFAL
710 720 730 740 750
PYEPPPEAPD KEYPFWLSTG RVLEHWHSGS MTRRVPELYK AFPEAVCFMH
760 770 780 790 800
PDDAQALGVR RGVEVEVVSR RGRMRTRVET RGRDKPPRGL VFVPWFDAGQ
810 820
LINKVTLDAT DPISFQTDFK KCAVKIVKV
Length:829
Mass (Da):92,970
Last modified:October 1, 2003 - v1
Checksum:i7E55710BACD97B3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640445 Genomic DNA Translation: CAE33292.1

Genome annotation databases

EnsemblBacteriaiCAE33292; CAE33292; BB2800
KEGGibbr:BB2800

Similar proteinsi

Entry informationi

Entry nameiNAPA_BORBR
AccessioniPrimary (citable) accession number: Q7WIQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2003
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health