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Q7WIQ1

- NAPA_BORBR

UniProt

Q7WIQ1 - NAPA_BORBR

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciBBRO257310:BB2800-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:BB2800
    OrganismiBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
    Taxonomic identifieri257310 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
    ProteomesiUP000001027: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3636Tat-type signalUniRule annotationAdd
    BLAST
    Chaini37 – 829793Periplasmic nitrate reductasePRO_0000045977Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi257310.BB2800.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7WIQ1.
    SMRiQ7WIQ1. Positions 39-826.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHPKTRVA.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q7WIQ1-1 [UniParc]FASTAAdd to Basket

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    MARRDFIKQT AAAAAATVAG VPLTGYTQNI VTESEAAKLK WSKAPCRFCG    50
    TGCGVNVAVK DNQVVATHGD FNAEVNKGLN CVKGYFLSKI MYGSDRLTQP 100
    LLRMKDGKYA KDGEFAPVSW DQAFDVMAEQ FKRVLKDKGP EAVGMFGSGQ 150
    WTVWEGYAAL KLMKAGFRTN NLDPNARHCM ASAAVGFMRT FGADEPMGCY 200
    DDIENADAFV LWGSNMAEMH PILWTRVTDR RLSAPATKVA VLSTFEHRSY 250
    ELADLTLTFE PQSDLAILNY IANHIIRTKR VNRDFVDKHT VFREGNADIG 300
    YGLRPEHPLQ QAARNAGDAG GSKPITFDDF ARFVSKYDLE YTAKLSGVPK 350
    NRLEELAELY ADPKVRVTSF WTMGFNQHTR GVWCNNMVYN IHLLTGKIST 400
    PGNSPFSLTG QPSACGTARE VGTFSHRLPA DLVVTNPEHR RHAEEIWKLP 450
    DGTIPSKVGA HAVLQNRMLK DGKINAYWVM VNNNMQAAAN LMNEGLPGYR 500
    NPENFIVVSD AYPTVTTLSA DLILPAAMWV EKEGAYGNAE RRTQFWHQLV 550
    DAPGQARSDL WQLVEFSKRF KVEEVWPADL LAKKPEYRGK TLYDVLFANG 600
    KVNQFPNTEL DAEYANQEAQ AFGFYLQKGL FEEYAEFGRG HGHDLAPFDV 650
    YHKARGLRWP VVDGKETLWR YREGSDPYVK PGTGFQFYGN PDGKAVIFAL 700
    PYEPPPEAPD KEYPFWLSTG RVLEHWHSGS MTRRVPELYK AFPEAVCFMH 750
    PDDAQALGVR RGVEVEVVSR RGRMRTRVET RGRDKPPRGL VFVPWFDAGQ 800
    LINKVTLDAT DPISFQTDFK KCAVKIVKV 829
    Length:829
    Mass (Da):92,970
    Last modified:October 1, 2003 - v1
    Checksum:i7E55710BACD97B3A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX640445 Genomic DNA. Translation: CAE33292.1.
    RefSeqiNP_889336.1. NC_002927.3.

    Genome annotation databases

    EnsemblBacteriaiCAE33292; CAE33292; BB2800.
    GeneIDi2661370.
    KEGGibbr:BB2800.
    PATRICi21138941. VBIBorBro124907_2839.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX640445 Genomic DNA. Translation: CAE33292.1 .
    RefSeqi NP_889336.1. NC_002927.3.

    3D structure databases

    ProteinModelPortali Q7WIQ1.
    SMRi Q7WIQ1. Positions 39-826.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257310.BB2800.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE33292 ; CAE33292 ; BB2800 .
    GeneIDi 2661370.
    KEGGi bbr:BB2800.
    PATRICi 21138941. VBIBorBro124907_2839.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HPKTRVA.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci BBRO257310:BB2800-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica."
      Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S.
      , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J.
      Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-588 / NCTC 13252 / RB50.

    Entry informationi

    Entry nameiNAPA_BORBR
    AccessioniPrimary (citable) accession number: Q7WIQ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3