Reviewed, UniProtKB/Swiss-Prot Q7WI34 (GCP_BORBR)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BB3022

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable. HAMAP MF_01445
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303285
Sites
	Metal binding	111	1	Zinc Potential
	Metal binding	115	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7WI34-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: A44ACB23660C085C	FASTA	346	36,197
	10 20 30 40 50 60 MIILGFESSC DETGVAAVCT ERGLLAHALH TQIAMHQEYG GVVPELASRD HIRRVVPLTR 70 80 90 100 110 120 QVLAEAGLTL ADVGAVAYTA GPGLAGALLV GASVAQALAW SRALPAIGIH HLEGHLLSPL 130 140 150 160 170 180 LAEPRPEFPF VALLVSGGHT QLMRVDGVGR YELLGETLDD AAGEAFDKSA KLMGLGYPGG 190 200 210 220 230 240 PALARLAEQG DASRYDLPRP MLHSGDLDFS FSGLKTAVLT RVKAATRDGG ELGEQDRADL 250 260 270 280 290 300 AAATQAAIVE VLAAKAIRAL KQTGLRRLVV AGGVGANALL RAHLARALKP LRAEAYFPPL 310 320 330 340 SLCTDNGAMI AFAAAERVKA GLADLREGDH AFTVRPRWDL ADIQAG

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640446 Genomic DNA. Translation: CAE33514.1.
RefSeq	NP_889558.1.
3D structure databases
SMR	Q7WI34. Positions 1-345.
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	2660721.
KEGG	bbr:BB3022.
NMPDR	fig|257310.1.peg.3005.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304663.
OMA	CKRALKQ.
PhylomeDB	Q7WI34.
Enzyme and pathway databases
BioCyc	BBRO257310:BB3022-MONOMER.
BRENDA	3.4.24.57. 413.
Family and domain databases
HAMAP	MF_01445. Glycoptase_bact. [Tree]
InterPro	IPR009180. Pept_M22_O-sialoglycoprot. IPR000905. Peptidase_M22. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BORBR

Accession

Primary (citable) accession number: Q7WI34

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)