Reviewed, UniProtKB/Swiss-Prot Q7WHW8 (DNAE2_BORBR)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	BB3088

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	1052 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1052	1052	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103367

Sequences

	Sequence		Length	Mass (Da)
	Q7WHW8-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D95FADC2CA5D656F	FASTA	1,052	115,105
	10 20 30 40 50 60 MSAILPGYAE LHCQSNFSFL QGASHPEELV TRAGELGYAA LALTDECSLA GVVRAHVEAR 70 80 90 100 110 120 EQKLPLIIGS SFTLQAGADA PPLDLTLLAQ NREGYGNLAE LITLGRGRAA KGQYLLTPAD 130 140 150 160 170 180 IEAPAGDNAH LRGMPDCLAI LAPPPGLAAE RLAEQARWLA AQCPGRAWIG LTLLHHCRDD 190 200 210 220 230 240 LHRAAVEHAA HASGLPIVAL GQAQMHRRSR KPLHDTLAAI RTGRSVGQCG YDLAANAERH 250 260 270 280 290 300 LRSRLRLASL YPAQALAQTL AIARRCTFSL DELQYEYPDE IVPAGHTPAS YLRQQTYLGA 310 320 330 340 350 360 RQRFPEGMTP AVAAQVEKEL ALINELRYEA YFLTVYDIVG YARSQGILCQ GRGSAANSAV 370 380 390 400 410 420 CYCLGITAVD PARGNTLFER FISKERNEPP DIDVDFEHQR REEVIQYIYE KYGRQRAALT 430 440 450 460 470 480 AVVISYRPRS VLRDTGRALG VDNGIIDAVA RAHQWWDGKK EMLRSLAACG LDPASRVARQ 490 500 510 520 530 540 WAELAETLMG FPRHLSQHPG GFVISRGKLS RLVPIENAAM PGRSVVQWDK DDLDALRLLK 550 560 570 580 590 600 VDVLALGMLS VLRRALALAG QRRGRPLALH EIPPDDDATY DMICAADTIG VFQIESRAQM 610 620 630 640 650 660 SMLPRLRPRQ YYDLVVQVAI VRPGPIQGGM VHPYLRRRQG REDITYPGPA VRKALARTLG 670 680 690 700 710 720 VPIFQEQVMQ IAVDAAGFTP GEADALRRSM AAWRRKGGVD KFRAQLVGGL LARNYTADFA 730 740 750 760 770 780 QALFRQIEGF GEYGFPESHA ASFALLAYAS SWLKCHEPEA FLAALLNSQP MGFYAPAQLV 790 800 810 820 830 840 QDARRHGVRV LPADVLYSGW EASLQDAPGA ARPAVRLGLN LVKGLREDSA RAIEQARVRR 850 860 870 880 890 900 PFADTADMAR RAGLPRQALD ALAAADALRT LAGHRRLASW QAAASAQSRD LLREAVIVET 910 920 930 940 950 960 ETPALPAPSE GQTVAADYRS VGLTLGRHPL ALLRPQLAAR NFQTAAVLNT YPNRRLARAC 970 980 990 1000 1010 1020 GIVTVRQRPQ TAKGTIFVTL EDETGPINAV VRPELIERQR RELLDATLLG IYGTWQSVDG 1030 1040 1050 VRHLVAQRLV DLSSLLGQLS QDGLAAASRN FH

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640446 Genomic DNA. Translation: CAE33580.1.
RefSeq	NP_889624.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2663274.
GenomeReviews	Gene locus BB3088 in contig BX470250_GR.
KEGG	bbr:BB3088.
NMPDR	fig|257310.1.peg.3071.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7WHW8.
OMA	Q7WHW8. MGFYSPD.
Enzyme and pathway databases
BioCyc	BBRO257310:BB3088-MON.
BRENDA	2.7.7.7. 413.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_BORBR

Accession

Primary (citable) accession number: Q7WHW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)