Reviewed, UniProtKB/Swiss-Prot Q7WHL6 (SYA_BORBR)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Synonyms:	lovB
Ordered Locus Names:	BB3191

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm HAMAP MF_00036.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075069

Sequences

	Sequence		Length	Mass (Da)
	Q7WHL6-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 7FEB78F43474C51D	FASTA	874	94,867
	10 20 30 40 50 60 MKSSEIRQKF LQFFQSKGHT IVPSSSLVPA NDPTLLFTNS GMVQFKDVFT GKEARAYKRA 70 80 90 100 110 120 TSSQRSVRAG GKHNDLENVG YTARHHTFFE MLGNFSFGDY FKREAIQYAW ELLTQVYRLP 130 140 150 160 170 180 AEKLWVTVYQ EDDEAYDIWA KEVGVPAERI IRIGDNKGAR YASDNFWQMA DTGPCGPCSE 190 200 210 220 230 240 IFYDHGPEIW GGPPGSPEED GDRYIEIWNL VFMQFERDAA GNMERLPKPC VDTGMGLERI 250 260 270 280 290 300 AAVLQHVHSN YEIDLFQKLI AAAARETGVK DLADNSLKVI ADHIRACAFL IVDGIIPSNE 310 320 330 340 350 360 GRGYVLRRIV RRALRHGYKL GQTKPFFHRL VPDLVAEMGE AYPELAQVAE RVAQVLRQEE 370 380 390 400 410 420 ERFGETLEHG MKILDGALAK VAKGDPLDGT TLFTLYDTYG FPVDLTADIC RERGVEVDMA 430 440 450 460 470 480 GFEAAMQRQR EQARAAGKFK MAEGLSYEGA ETRFEGYESL ELSGVKVTAL YVEGTQVEQV 490 500 510 520 530 540 SAGQDAVVVL DATPFYAESG GQVGDTGLLE AGGVRFAVAD TLKIQPGVFG HHGTLEAGAL 550 560 570 580 590 600 KVGDTLLARV DAVRRARTVR NHSATHLMHK ALREVLGAHV QQRGSLVDPD KTRFDFAHDA 610 620 630 640 650 660 PMTAEQIARV EAIVNAEVLA NQATEAKVMA YDDAVKGGAM ALFGEKYGDT VRVLDIGFSR 670 680 690 700 710 720 ELCGGTHVRR TGDIGLFKVV SEGGVAAGVR RIEAITGDNA LAWVQDQNAL LQRAAGVLRA 730 740 750 760 770 780 PAHELPERIV QVQEQLKALE KELEQARTKL AASAGNDLAA TATVEVKGIK VLAASIGDVD 790 800 810 820 830 840 PKALRGMVDN LKDRLKPAVV LLAAGSADGK ISLVGGVTAD LTGRIKAGDL VGFVAGQVGG 850 860 870 KGGGRPDMAM GGGTDLAALP AAVASVQKWV DERL

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640446 Genomic DNA. Translation: CAE33683.1.
RefSeq	NP_889727.1.
3D structure databases
SMR	Q7WHL6. Positions 1-460, 486-694.
ModBase	Search...
Genome annotation databases
GeneID	2660548.
KEGG	bbr:BB3191.
NMPDR	fig|257310.1.peg.3174.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
OMA	TLMFTNS.
PhylomeDB	Q7WHL6.
Enzyme and pathway databases
BioCyc	BBRO257310:BB3191-MONOMER.
BRENDA	6.1.1.7. 413.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BORBR

Accession

Primary (citable) accession number: Q7WHL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)