Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:BB3233
OrganismiBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Taxonomic identifieri257310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000001027 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092311 – 941Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST941

Interactioni

Protein-protein interaction databases

STRINGi257310.BB3233.

Structurei

3D structure databases

ProteinModelPortaliQ7WHH4.
SMRiQ7WHH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 431Adenylyl removaseUniRule annotationAdd BLAST431
Regioni447 – 941Adenylyl transferaseUniRule annotationAdd BLAST495

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256491.
KOiK00982.
OMAiFTVQLLQ.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q7WHH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAPPFAAA LAWSGHLRRR LDAHPDLAAW LANACAHPVS ANVLAAWQAE
60 70 80 90 100
LSGPDAPEVL PVEQCRGMLR KLRERVFLTL IVRDLGGQAD LEEVVGAMTV
110 120 130 140 150
LADIAVGTAY RSVAAELAAV HGLPREQSTG DPQEMLIVGM GKLGGRELNV
160 170 180 190 200
SSDIDLVMLY GDEGETDGPR RISNHEFYGR LTRRMMPVLS EVDADGQVFR
210 220 230 240 250
TDLRLRPDGD AGPLAWSLDA LEHYLIGQGR EWERYAWLKA RLMPAQAFAD
260 270 280 290 300
SNPDAQARQL ESLRVPFVYR KYFDFDALAA LRALRERIRQ DWQRRALARN
310 320 330 340 350
GVDSANNIKL GDGGIREIEF IVQLSQLIRG GRMPALQRRG LLEALHAERA
360 370 380 390 400
AGLVPEGDAQ KLEAAYRFLR RTEHALQYRE DEQTHLLPAD PAQRAALAAA
410 420 430 440 450
LGYEPAAFER TLAEHRAFVS QTFRNAFRLA GMGEEDDSPA PARTPANGHG
460 470 480 490 500
MRPHAGALHD IEERLAGQIQ RDFPEHAEDL LRRTETLLGS HRVRSLPDSS
510 520 530 540 550
RHRLEALLPA ALTAAAQTSA PMDAALRLFD LIETIAQRSA YLALLAEYPD
560 570 580 590 600
TLARVARMVA ASPWAAQYLT QHPLLLDSLI DWRTLFEPLD FAQVARQLAA
610 620 630 640 650
DLDACRLPDG EPDIERQMNL MRDVQRQASF QLLAQDLEGE LTVEKLADQL
660 670 680 690 700
SALADLLLAE TIRRVWPLVN RRPGAEPHLA VIAYGKLGGK ELGYASDLDL
710 720 730 740 750
VFLFDDDRED AAELYAKLGR RMTSWLSTMT SSGRLYEVDL RLRPDGDAGL
760 770 780 790 800
LAVSLEAFEQ YQRSHAWPWE HQALTRARYA AGDTEAGARF ERIRADILVM
810 820 830 840 850
PRDVQALRGE VLGMRDKISA GHPNRSELFD VKHDRGGMVD VEFVTQYLVL
860 870 880 890 900
CHAATHRVLV NNLGNIALLR LAGEAGLIPA PLALAAGDAY RTLRRAQHQL
910 920 930 940
RLKGVDKARV PPGQLAAERA TVCELWQTVL QDGTIAQAEV K
Length:941
Mass (Da):104,306
Last modified:October 1, 2003 - v1
Checksum:iA87412583824BFBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640446 Genomic DNA. Translation: CAE33725.1.
RefSeqiWP_003810574.1. NC_002927.3.

Genome annotation databases

EnsemblBacteriaiCAE33725; CAE33725; BB3233.
KEGGibbr:BB3233.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLNE_BORBR
AccessioniPrimary (citable) accession number: Q7WHH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2003
Last modified: June 7, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families