Reviewed, UniProtKB/Swiss-Prot Q7WFS0 (MURD_BORBR)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BB4200

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108976
Regions
	Nucleotide binding	138 – 144	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7WFS0-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 6CDBB564A1B57FFA	FASTA	510	54,006
	10 20 30 40 50 60 MNTTETSRAA APLVLILGLG ETGVAAARWC ARQGSPLRVA DTRAQPGGLA ALQAALADAT 70 80 90 100 110 120 VEYRLGCGEQ FPPDLLDGVA QIVLSPGLVP HESPTRELLE QARERNVEVV GEIELFARAL 130 140 150 160 170 180 AGLAESREYR PRVLAITGTN GKTTVTALTR QLIEAGGMSA RAAGNISPAA LAALIDALDQ 190 200 210 220 230 240 DDLPQVWVLE LSSFQLETTR TLAPDAAVVL NVTQDHLDWH GDMQAYAQAK ARILKPARLA 250 260 270 280 290 300 IVNRDDPLTV AMVESLQALN VRSFGRDVPA LVGDMGLELG QGVAWLTACE SNDFDEPAPA 310 320 330 340 350 360 PRRKKDAPPP TRAGGRMSRL MPVDALRIRG VHNALNALAA MQLARSLDLG WGPMLRTLRD 370 380 390 400 410 420 YAGEPHRAEL VRSIGDVDYI NDSKGTNVGA TVAALEGLGQ QVVLIAGGQG KGQDFSPLVP 430 440 450 460 470 480 VVRRHARAVV LIGVDGAAIG KVLEPTGVPC VAAADMREAV RRAAELAQPG DAVLLSPACA 490 500 510 SFDMFRNYPH RGEVFAAEVQ ELALDRGEVA

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640449 Genomic DNA. Translation: CAE34564.1.
RefSeq	NP_890735.1.
3D structure databases
HSSP	HSSP built from PDB template 1E0D based on UniProtKB P14900.
ModBase	Search...
Genome annotation databases
GeneID	2661413.
GenomeReviews	Gene locus BB4200 in contig BX470250_GR.
KEGG	bbr:BB4200.
NMPDR	fig|257310.1.peg.4182.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7WFS0.
OMA	SEDHLDR.
Enzyme and pathway databases
BioCyc	BBRO257310:BB4200-MON.
BRENDA	6.3.2.9. 413.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BORBR

Accession

Primary (citable) accession number: Q7WFS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)