Reviewed, UniProtKB/Swiss-Prot Q7WFQ5 (ILVD3_BORBR)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 3
      Short name=DAD 3
    EC=4.2.1.9

Gene names

Name:	ilvD3
Ordered Locus Names:	BB4216

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Dihydroxy-acid dehydratase 3 HAMAP MF_00012		PRO_0000103436
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7WFQ5-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 328318406E4AB0E1	FASTA	561	59,657
	10 20 30 40 50 60 MSDNNRSRHI TEGVARAPNR AMYYALGYTE ADFQNPMIGV ANGHSTITPC NSGLQRLADA 70 80 90 100 110 120 AIEAIRVSRA NPQVFGTPTI SDGMSMGTEG MKYSLVSREV IADCIETAAQ GQWMDGVVVI 130 140 150 160 170 180 GGCDKNMPGG MMALARMNVP GIYVYGGTIK PGHYKGKDLT IVSVFEAVGE YTMGRMDETD 190 200 210 220 230 240 FKAIEQCAIP GSGSCGGMYT ANTMSSAFEA MGMSLPHSST MANEDQEKVA SAAESARVLV 250 260 270 280 290 300 EAVRRQLRPR DIITRASIEN AVAVIMATGG STNAVLHFLA IAHAAEVPWN IDDFERIRKR 310 320 330 340 350 360 VPVICDLKPS GKYVATDLHR AGGIPQVMKI LLNAGLLHGD CITITGKTVA ETLANVPDAP 370 380 390 400 410 420 PPGQDVIMPI ERALYPQGHL AILKGNLSPE GCVAKITGLK NPVITGPARV FDSEDDAMSA 430 440 450 460 470 480 IMDRRIRDGD VVVIRYEGPK GGPGMREMLA PTSALVGQGL GETVGLITDG RFSGGTWGMV 490 500 510 520 530 540 VGHVAPEAFV GGPIALIREG DSVTIDAHQL LLQLNISDEE MAARRKAWAQ PKPRYVRGVL 550 560 AKFGKLACTA SRGAVTDAFE E

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640449 Genomic DNA. Translation: CAE34580.1.
RefSeq	NP_890751.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2662002.
GenomeReviews	Gene locus BB4216 in contig BX470250_GR.
KEGG	bbr:BB4216.
NMPDR	fig|257310.1.peg.4198.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7WFQ5.
OMA	QVMKILL.
Enzyme and pathway databases
BioCyc	BBRO257310:BB4216-MON.
BRENDA	4.2.1.9. 413.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD3_BORBR

Accession

Primary (citable) accession number: Q7WFQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)