Reviewed, UniProtKB/Swiss-Prot Q7WFD2 (GLYA2_BORBR)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 2
      Short name=Serine methylase 2
      Short name=SHMT 2
    EC=2.1.2.1

Gene names

Name:	glyA2
Ordered Locus Names:	BB4348

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Serine hydroxymethyltransferase 2 HAMAP MF_00051		PRO_0000113538
Amino acid modifications
	Modified residue	229	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WFD2-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 59962EB7C7E9C5BE	FASTA	415	44,811
	10 20 30 40 50 60 MFNRNLTLDQ VDPDVWAAIQ KEDVRQEQHI ELIASENYAS PAVMQAQGTQ LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEY VDVVEQLAID RLKQIFGAEA ANVQPNSGSQ ANQGVYMAVL KPGDTVLGMS 130 140 150 160 170 180 LAEGGHLTHG ASVNASGKLY NFVPYGLDAD EVLDYAQVER LTKEHKPKLI VAGASAYALH 190 200 210 220 230 240 IDFERMARIA HDNGALFMVD IAHYAGLVAG GAYPNPVPHA DFVTSTTHKS LRGPRGGVIM 250 260 270 280 290 300 MKAEFEKAVN SAIFPGIQGG PLMHVIAAKA VAFKEALSPE FQDYAQQVVK NAKVLADTLV 310 320 330 340 350 360 KRGLRIVSGR TESHVMLVDL RPKGITGKEA EAVLGQAHIT VNKNAIPNDP EKPFVTSGIR 370 380 390 400 410 LGTPAMTTRG FKEAEAELTA NLIADVLDNP RDEANIAAVR ARVNELTARL PVYGN

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640450 Genomic DNA. Translation: CAE34711.1.
RefSeq	NP_890882.1.
3D structure databases
SMR	Q7WFD2. Positions 1-414.
ModBase	Search...
Genome annotation databases
GeneID	2659781.
KEGG	bbr:BB4348.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG301263.
OMA	WTAIQDE.
PhylomeDB	Q7WFD2.
Enzyme and pathway databases
BioCyc	BBRO257310:BB4348-MONOMER.
BRENDA	2.1.2.1. 413.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_BORBR

Accession

Primary (citable) accession number: Q7WFD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)