Reviewed, UniProtKB/Swiss-Prot Q7WF83 (AROE_BORBR)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	BB4397

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Shikimate dehydrogenase HAMAP MF_00222		PRO_0000325106
Regions
	Nucleotide binding	132 – 136	5	NADP By similarity
Sites
	Active site	71	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7WF83-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 9FE2BFF01392F074	FASTA	287	29,576
	10 20 30 40 50 60 MTLPASPPRY AVIGNPIAHS RSPQIHAMFS AQTGRPLRYE RLLAPVDGFL PTVQAFRESG 70 80 90 100 110 120 GLGLNVTVPF KLEAYALAEA RLSERARLAG AVNTLSWRDG AWHGCNTDGV GLVNDLLRLG 130 140 150 160 170 180 VALAGARVLL VGAGGAARGV LQPLAAAGCA RIHIVNRTAA RAAELAAAWR AAAPRTGTQV 190 200 210 220 230 240 SAGALAQAAE PGGWDVAINA TASGLQDAAP DLPGGLYAPD ALAYDMMYGA RPTAFMRQAE 250 260 270 280 ADGAARCADG LGMLVGQAAE SFHIWHGVRP DPGPVLLALR TELLAAG

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640450 Genomic DNA. Translation: CAE34760.1.
RefSeq	NP_890931.1.
3D structure databases
HSSP	HSSP built from PDB template 1NYT based on UniProtKB P15770.
SMR	Q7WF83. Positions 9-283.
ModBase	Search...
Genome annotation databases
GeneID	2660716.
KEGG	bbr:BB4397.
NMPDR	fig|257310.1.peg.4378.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553408.
OMA	DQYVVFG.
PhylomeDB	Q7WF83.
Enzyme and pathway databases
BioCyc	BBRO257310:BB4397-MONOMER.
BRENDA	1.1.1.25. 413.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_BORBR

Accession

Primary (citable) accession number: Q7WF83

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)