Reviewed, UniProtKB/Swiss-Prot Q7WF38 (GLPK_BORBR)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	BB4442

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Glycerol kinase HAMAP MF_00186		PRO_0000059435
Regions
	Nucleotide binding	415 – 419	5	ATP By similarity
Sites
	Binding site	14	1	Substrate By similarity
	Binding site	18	1	ATP By similarity
	Binding site	84	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	267	1	ATP By similarity
	Binding site	314	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WF38-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: EF62A75C47196B48	FASTA	508	54,652
	10 20 30 40 50 60 MTANEFILAL DQGTTSSRAI VFDRAGTVRG MGQREFRQHY PRPGWVEHDA GEIWQSQLEV 70 80 90 100 110 120 AREALRNAGA SAADLAALGI TNQRETTLIW ERATGRPLAR AIVWQDRRTA AMCEKLLHDG 130 140 150 160 170 180 HGRMLQERTG LVVDAYFSGT KLAWLLDHVP GARKMAERGE LAFGTVDTWL VWQLTGGAVH 190 200 210 220 230 240 STDPSNASRT MLFDLHAQDW SDDILALLNI PRGILPRIAP SSARIGETLP EWLGGSIPIA 250 260 270 280 290 300 GVAGDQQAAT FGQACFTPGM AKNTYGTGCF MLMNVGDAPV ASRHNLLSTV GWSLPAGNAT 310 320 330 340 350 360 HATYMVEGGV FMAGAAVQWL RDGLGIIQRS ADIEALAASV ADTDDVFMVP AFAGLGAPHW 370 380 390 400 410 420 DPYARGTLVG MTRGTTRAHI ARATLESIAL QSAELLSCMN ADSGIPLSEL RVDGGAARND 430 440 450 460 470 480 LLMQMQADLL GVPVVRPRVP ESTALGAAGL AGLAVGFWSS LDEFGAQWQA ERTFEPAWPA 490 500 DVREARMQRW RQAVELSKGW SRPAAGHA

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640450 Genomic DNA. Translation: CAE34805.1.
RefSeq	NP_890976.1.
3D structure databases
SMR	Q7WF38. Positions 6-502.
ModBase	Search...
Genome annotation databases
GeneID	2661068.
KEGG	bbr:BB4442.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	KPSSEVY.
PhylomeDB	Q7WF38.
Enzyme and pathway databases
BioCyc	BBRO257310:BB4442-MONOMER.
BRENDA	2.7.1.30. 413.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BORBR

Accession

Primary (citable) accession number: Q7WF38

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)