Q7WDP2 (ACEK_BORBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	BB4946

Organism

Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

257310 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000057894
Regions
	Nucleotide binding	354 – 360	7	ATP By similarity
Sites
	Active site	409	1	By similarity
	Binding site	375	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WDP2 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: F434CC157EFD9CB4	FASTA	619	70,681
	10 20 30 40 50 60 MIYSGDVQRI EPAPVAGPAP LDVAHLILAG FDRHYALFRY SAQRAKSLFE SGDWHGMQRL 70 80 90 100 110 120 SRERIEYYDM RVRECATQLD SALRGSDART ADGSRANGSA ALSEAQTAFW QAVKQEFVGL 130 140 150 160 170 180 LADHRQPECA ETFFNSVSCR ILHRDYFHND FLFVRPAIAT DYLDSRIPSY RVYYPVAEGL 190 200 210 220 230 240 HKSLIRMVAD FGLAVPYADL PRDARLLARA AVRQLRGQLP RHAGPRLASD CQIQVLGSLF 250 260 270 280 290 300 FRNTGAYIVG RLINQGTVYP FAVALRRNPA GQVCLDALLL GADDLSTLFS FTRAYFLVDM 310 320 330 340 350 360 ETPAAVVNFL ASLLPRKPKA ELYTMLGLQK QGKTLFYRDF LHHLTHSRDA FDIAPGIRGM 370 380 390 400 410 420 VMCVFTLPSY PYVFKLIKDR IDKDGMDHAT VRRKYQMVKL HDRVGRMADT WEYSQVALPR 430 440 450 460 470 480 SRFAPRLLEE LRRLVPSLIE ENGDTVVIRH VYIERRMMPL NLYLRHASDP LLEVAVREYG 490 500 510 520 530 540 DAIRQLATAN IFPGDMLYKN FGVTRLGRVV FYDYDEIQRM TEMNFRAIPP APNEEAELSS 550 560 570 580 590 600 EPWYAVGPND VFPEEFGRFL LGDPRVRQAF LRHHADLLAP QWWQACRARV AQGRIEEFFP 610 YDTDRRLHPQ AAPPPRTAA

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-588 / NCTC 13252 / RB50.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640452 Genomic DNA. Translation: CAE35310.1.
RefSeq	NP_891480.1. NC_002927.3.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2659899.
GenomeReviews	Gene locus BB4946 in contig BX470250_GR.
KEGG	bbr:BB4946.
NMPDR	fig|257310.1.peg.4927.
PATRIC	21143398. VBIBorBro124907_5040.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298593.
OMA	RMTPLNI.
PhylomeDB	Q7WDP2.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	BBRO257310:BB4946-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_BORBR

Accession

Primary (citable) accession number: Q7WDP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program