Reviewed, UniProtKB/Swiss-Prot Q7WD42 (AMPA_BORBR)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BB3736

Organism

Bordetella bronchiseptica (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]

Taxonomic identifier

518 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165724
Sites
	Active site	283	1	Potential
	Active site	357	1	Potential
	Metal binding	271	1	Manganese 2 By similarity
	Metal binding	276	1	Manganese 1 By similarity
	Metal binding	276	1	Manganese 2 By similarity
	Metal binding	294	1	Manganese 2 By similarity
	Metal binding	353	1	Manganese 1 By similarity
	Metal binding	355	1	Manganese 1 By similarity
	Metal binding	355	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7WD42-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: EEBD5807F9BFB5C5	FASTA	499	51,729
	10 20 30 40 50 60 MEFSTQTTAS LHQIKTAALA VGVFADGVLS AAAEVIDRAS HGAVAAVVKS EFRGRTGSTL 70 80 90 100 110 120 VLRSLAGVSA QRVVLVGLGK QAEYNARAHA SAEQAFAAAC VAAQVGEGVS TLAGVAIEGV 130 140 150 160 170 180 PVRARARSAA IAAGAAAYHY DATFGKANRD ARPRLKKIVQ VVDRAASAQA QLGLREGAAI 190 200 210 220 230 240 AHGMELTRTL GNLPGNVCTP AYLGNTAKKL AREFKSLKVE VLERKQVEAL GMGSFLSVAR 250 260 270 280 290 300 GSEEPLRFIV LRHAGKPAKK DKAGPVVLVG KGITFDAGGI SLKPAATMDE MKYDMCGAAS 310 320 330 340 350 360 VLGTFRALAE LELPLDVVGL IAACENLPSG KANKPGDVVT SMSGQTIEIL NTDAEGRLVL 370 380 390 400 410 420 CDALTYAERF KPAAVIDIAT LTGACVVALG NVNSGLFSKD DALADALLAA SRQSLDPAWR 430 440 450 460 470 480 LPLDDAYQDQ LKSNFADIAN IGGPPAGAVT AACFLSRFTK AYPWAHLDIA GTAWRGGKDK 490 GATGRPVPLL MQYLLDQAG

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RB50 / ATCC BAA-588 / NCTC 13252.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640448 Genomic DNA. Translation: CAE35710.1.
RefSeq	NP_890271.1.
3D structure databases
HSSP	HSSP built from PDB template 1GYT based on UniProtKB P11648.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	2661335.
GenomeReviews	Gene locus BB3736 in contig BX470250_GR.
KEGG	bbr:BB3736.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7WD42.
OMA	LGHHNSG.
Enzyme and pathway databases
BioCyc	BBRO257310:BB3736-MON.
BRENDA	3.4.11.1. 413.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BORBR

Accession

Primary (citable) accession number: Q7WD42

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)