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Q7WCJ7 (DNLJ_BORBR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name:ligA
Ordered Locus Names:BB3804
OrganismBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus) [Complete proteome] [HAMAP]
Taxonomic identifier257310 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length696 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity. HAMAP MF_01588

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA ligase (NAD+) activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 696696DNA ligase HAMAP MF_01588
PRO_0000313144

Regions

Domain618 – 69679BRCT
Nucleotide binding36 – 405NAD By similarity
Nucleotide binding85 – 862NAD By similarity

Sites

Active site1251N6-AMP-lysine intermediate By similarity
Metal binding4371Zinc By similarity
Metal binding4401Zinc By similarity
Metal binding4551Zinc By similarity
Metal binding4611Zinc By similarity
Binding site1231NAD By similarity
Binding site1461NAD By similarity
Binding site1811NAD By similarity
Binding site3191NAD By similarity
Binding site3431NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7WCJ7 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: CBB4177DB1837EA5

FASTA69675,556
        10         20         30         40         50         60 
MAQAGPTPQQ AIARLRAEIE QHNIRYYVHD DPSVPDAEYD ALMRDLQALE AEHPELVTPD 

        70         80         90        100        110        120 
SPTQRVGAAP LAEFGSVRHA VPMLSLGNAF DEEDVRAFDK RVADTLRGAG LLGLDQQVEY 

       130        140        150        160        170        180 
FCELKLDGLA ISLRYEEGRL AQAATRGDGQ TGEDVTANIR TIKGVPLRLH GAPRVLEVRG 

       190        200        210        220        230        240 
EVLMNRAEFE RLNRTQAARG EKVFVNPRNA AAGSLRQLDP RITAQRPLRF FAYSWGEVHG 

       250        260        270        280        290        300 
LPEGMPTRFD EPAPGVRVAS TLPRDTHGGM LDWLAELGLP VNLRHNHRER GADGLLAFYE 

       310        320        330        340        350        360 
RIGKLRADLP YDIDGVVYKV DALPSQRVLG FVARAPRFAL AHKFPAEEAV TQLLGIEVQV 

       370        380        390        400        410        420 
GRTGAITPVA RLAPVFVGGV TVTNATLHNE DEIRRKDVRI GDTVIVRRAG DVIPEVVGPV 

       430        440        450        460        470        480 
LEKRPADARE FVMLTACPIC GSAIERPEGE AIARCTGGLF CAAQRKQTLL HAAGRKALDI 

       490        500        510        520        530        540 
EGLGEKLIDQ LVDADRVKSL ADIYSLTAFE LAALERMGKK SAENLVAAID QARRPALGRL 

       550        560        570        580        590        600 
LFALGIRHVG ETTARDVARH FGSMERIMDA SEEALLAVPD VGGVVAGSIR RFFAEPHNRE 

       610        620        630        640        650        660 
IVEQLTQQGV HPQAEAEPEG TSLAGKTFVL TGTMPNWTRD EATRRILAAG GKVSGSVSKK 

       670        680        690 
TAYLVTGEDA GSKLTKAQEL GVPVLDEDGL KALLGL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX640448 Genomic DNA. Translation: CAE35778.1.
RefSeqNP_890339.1. NC_002927.3.

3D structure databases

HSSPHSSP built from PDB template 1B04 based on UniProtKB O87703.
ProteinModelPortalQ7WCJ7.
SMRQ7WCJ7. Positions 9-614.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2661129.
GenomeReviewsGene locus BB3804 in contig BX470250_GR.
KEGGbbr:BB3804.
NMPDRfig|257310.1.peg.3786.
PATRIC21140995. VBIBorBro124907_3858.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG620317.
OMAENVRTIR.
PhylomeDBQ7WCJ7.
ProtClustDBCLSK2516705.

Enzyme and pathway databases

BioCycBBRO257310:BB3804-MONOMER.

Family and domain databases

HAMAPMF_01588. DNA_ligase_A.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01972.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 2 hits.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00278. HhH1. 4 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. Dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_BORBR
AccessionPrimary (citable) accession number: Q7WCJ7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2003
Last modified: January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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