Reviewed, UniProtKB/Swiss-Prot Q7WC98 (ILVD1_BORPA)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 1
      Short name=DAD 1
    EC=4.2.1.9

Gene names

Name:	ilvD1
Ordered Locus Names:	BPP0429

Organism

Bordetella parapertussis [Complete proteome] [HAMAP]

Taxonomic identifier

519 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Dihydroxy-acid dehydratase 1 HAMAP MF_00012		PRO_0000103437
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	198	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7WC98-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: C2E2C4B963A0A995	FASTA	619	65,522
	10 20 30 40 50 60 MPHYRSRTST HGRNMAGARA LWRATGMKDG DFGKPIIAVV NSFTQFVPGH VHLRDLGALV 70 80 90 100 110 120 ASEIEAAGGV AKEFNTIAVD DGIAMGHGGM LYSLPSRELI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAAMRLNIPV VFVSGGPMEA GKITSPVDGK VIAKLDLVDA MIKAADPNVS 190 200 210 220 230 240 DAEAEEVERS ACPTCGSCSG MFTANSMNCL TEAIGLALPG NGTIVATHAW RKGLFEQAGR 250 260 270 280 290 300 LVVELCRRYY EQDDASVLPR SIATKSAFEN AMTLDVAMGG STNTVLHLLA AAQEAGVDFT 310 320 330 340 350 360 MSDIDRISRR VPCLCKAAPA TDKYHIEDVH RAGGILGILG ELGRADLLDL SCGNVHSGTL 370 380 390 400 410 420 GEAINQWDIN GGAGEAAQKF FRAAPGGIPT TVAFSQDATF LTLDMDRQTG CIRDKAHAYS 430 440 450 460 470 480 QDGGLAVLYG NLAEKGCIVK TAGVDESQWV FTGRARVFES QEDAVEGILG DRVQAGDVVI 490 500 510 520 530 540 IRYEGPKGGP GMQEMLYPTS YLKSKGLGKT CALFTDGRFS GGSSGLVIGH ASPEAAEGGT 550 560 570 580 590 600 IGLVEEGDTI EIDIPNRRIH LAVGDTVLAE RRAAMQARGE QAWQPVDRER VVSQALRAYA 610 ALATSADRGA VRDLSQLKR

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640424 Genomic DNA. Translation: CAE36013.1.
RefSeq	NP_882781.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1668601.
GenomeReviews	Gene locus BPP0429 in contig BX470249_GR.
KEGG	bpa:BPP0429.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7WC98.
OMA	SRKVPCL.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP0429-MON.
BRENDA	4.2.1.9. 263123.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD1_BORPA

Accession

Primary (citable) accession number: Q7WC98

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)