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Q7WC23 (TRMB_BORPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BPP0505
OrganismBordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253) [Complete proteome] [HAMAP]
Taxonomic identifier257311 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence caution

The sequence CAE36089.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171302

Regions

Region233 – 2364Substrate binding By similarity

Sites

Active site1621 By similarity
Binding site871S-adenosyl-L-methionine By similarity
Binding site1121S-adenosyl-L-methionine By similarity
Binding site1391S-adenosyl-L-methionine By similarity
Binding site1621S-adenosyl-L-methionine By similarity
Binding site1661Substrate By similarity
Binding site1981Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7WC23 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: 8E4A53B481039BE5

FASTA25428,065
        10         20         30         40         50         60 
MNTNTPAHPP EGAPLSEATQ AALASAEHAP DSPGATHIRS FVHRRGHITQ RQRDALEQLM 

        70         80         90        100        110        120 
GKWSVPYAPR PLDMAATFGR QAPTILEIGF GMGETTEKIA LARPGDNFLG VEVFNAGVGS 

       130        140        150        160        170        180 
LLHRIEESAI ANLRIVQHDA VEVVRDMIAP DSLAGVHVYF PDPWPKKRHH KRRLLQPPFV 

       190        200        210        220        230        240 
ALLASRLAPG GYLHCATDWE DYAVQMLEVL GGEPLLRNTA DGYAPRPDFR PQTKFETRGL 

       250 
RLGHGVWDLM FKRA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX640424 Genomic DNA. Translation: CAE36089.1. Different initiation.
RefSeqNP_882856.1. NC_002928.3.

3D structure databases

ProteinModelPortalQ7WC23.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257311.BPP0505.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE36089; CAE36089; BPP0505.
GeneID1668301.
KEGGbpa:BPP0505.
PATRIC21144593. VBIBorPar43418_0519.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycBPAR257311:BPP0505-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BORPA
AccessionPrimary (citable) accession number: Q7WC23
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: April 16, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways