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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87S-adenosyl-L-methionineUniRule annotation1
Binding sitei112S-adenosyl-L-methionineUniRule annotation1
Binding sitei139S-adenosyl-L-methionineUniRule annotation1
Active sitei162By similarity1
Binding sitei162S-adenosyl-L-methionineUniRule annotation1
Binding sitei166SubstrateUniRule annotation1
Binding sitei198SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:BPP0505
OrganismiBordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)
Taxonomic identifieri257311 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000001421 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001713021 – 254tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST254

Structurei

3D structure databases

ProteinModelPortaliQ7WC23.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni233 – 236Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073968.
KOiK03439.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7WC23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTNTPAHPP EGAPLSEATQ AALASAEHAP DSPGATHIRS FVHRRGHITQ
60 70 80 90 100
RQRDALEQLM GKWSVPYAPR PLDMAATFGR QAPTILEIGF GMGETTEKIA
110 120 130 140 150
LARPGDNFLG VEVFNAGVGS LLHRIEESAI ANLRIVQHDA VEVVRDMIAP
160 170 180 190 200
DSLAGVHVYF PDPWPKKRHH KRRLLQPPFV ALLASRLAPG GYLHCATDWE
210 220 230 240 250
DYAVQMLEVL GGEPLLRNTA DGYAPRPDFR PQTKFETRGL RLGHGVWDLM

FKRA
Length:254
Mass (Da):28,065
Last modified:July 19, 2004 - v2
Checksum:i8E4A53B481039BE5
GO

Sequence cautioni

The sequence CAE36089 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640424 Genomic DNA. Translation: CAE36089.1. Different initiation.
RefSeqiWP_033446655.1. NC_002928.3.

Genome annotation databases

EnsemblBacteriaiCAE36089; CAE36089; BPP0505.
KEGGibpa:BPP0505.
PATRICi21144593. VBIBorPar43418_0519.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640424 Genomic DNA. Translation: CAE36089.1. Different initiation.
RefSeqiWP_033446655.1. NC_002928.3.

3D structure databases

ProteinModelPortaliQ7WC23.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE36089; CAE36089; BPP0505.
KEGGibpa:BPP0505.
PATRICi21144593. VBIBorPar43418_0519.

Phylogenomic databases

HOGENOMiHOG000073968.
KOiK03439.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_BORPA
AccessioniPrimary (citable) accession number: Q7WC23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.