Reviewed, UniProtKB/Swiss-Prot Q7WA47 (LPXB_BORPA)

Names and origin

Protein names

Recommended name:
    Lipid-A-disaccharide synthase
    EC=2.4.1.182

Gene names

Name:	lpxB
Synonyms:	pgsB
Ordered Locus Names:	BPP1540

Organism

Bordetella parapertussis [Complete proteome] [HAMAP]

Taxonomic identifier

519 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity.
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP MF_00392
Pathway	Glycolipid biosynthesis; lipid (IV)A biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-D-glucosamine: step 5/6. HAMAP MF_00392
Sequence similarities	Belongs to the lpxB family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Lipid-A-disaccharide synthase HAMAP MF_00392		PRO_0000190153

Sequences

	Sequence		Length	Mass (Da)
	Q7WA47-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: C1482799F9CBC38C	FASTA	393	42,748
	10 20 30 40 50 60 MSLRIGMVAG EPSGDLLAGR IIAGLQARAP GVHCAGIGGP QMAARGFEAW HPMHALTVFG 70 80 90 100 110 120 YIDAFKRIPS LLSTYGDVKR RLLAEPPSVF VGIDAPDFNL RLEHQLRQAG TPTVHFVGPS 130 140 150 160 170 180 IWAWRYERIN KIRAAVSHML VLFPFEEALY RKEGIPVTYV GHPLAGVIPM QPDRAAARAR 190 200 210 220 230 240 LGIDADARVL AILPGSRSSE IRLLAPRFLQ AAAELVRRDP RLQCVVPMVN PQRRAEFEAI 250 260 270 280 290 300 AAQHPVPGLR CVTAAEGQGE TPVAWSVMEA SNAVLVASGT ATLETALYKR PMVISYVLSP 310 320 330 340 350 360 WMRRIMAWKS GQQRPYLPWV GLPNVLLRDF AVPELLQDEA TPAALAEATW QALTDEAGAA 370 380 390 RIEARFTALH QDLLRDTPAL AAQAILEVAD GAA

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640427 Genomic DNA. Translation: CAE36842.1.
RefSeq	NP_883830.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT19. Glycosyltransferase Family 19.
Genome annotation databases
GeneID	1668069.
GenomeReviews	Gene locus BPP1540 in contig BX470249_GR.
KEGG	bpa:BPP1540.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7WA47.
OMA	Q7WA47. DFTHRVA.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP1540-MON.
BRENDA	2.4.1.182. 263123.
Family and domain databases
HAMAP	MF_00392. [Tree]
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. lpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_BORPA

Accession

Primary (citable) accession number: Q7WA47

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)