Reviewed, UniProtKB/Swiss-Prot Q7W9M7 (PROA_BORPA)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	BPP1728

Organism

Bordetella parapertussis [Complete proteome] [HAMAP]

Taxonomic identifier

519 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Gamma-glutamyl phosphate reductase		PRO_0000189702

Sequences

	Sequence		Length	Mass (Da)
	Q7W9M7-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: B636D8727080BDEF	FASTA	419	44,321
	10 20 30 40 50 60 MSSIETYMQS VGEQTRTASR AMMRATGAAK NQALLAMAEA ILAQRAELQA ANAKDVAAAR 70 80 90 100 110 120 ANGLEAALLD RLTLSDRSIA LMAEGLRQIA ALPDPVGSIT ATSVRPNGMR VAQMRVPLGV 130 140 150 160 170 180 IGIIYESRPN VTIDAAALCL KSGNATILRG GSEALHSNVA LGRIVQAGLQ AAGLPTAAVQ 190 200 210 220 230 240 VIDTTDRAAV GKLVTMTEHV DVIVPRGGKG LISRLAQEAR VPLIKHLDGN CHVYVDAAAD 250 260 270 280 290 300 LAKAHDIAFN AKTYRYGVCG AMETLLVHAD VAQRLLPVLG QALHEHGVEL RGCARALQWL 310 320 330 340 350 360 PEGKPADDAD WATEYLGPIL AVRVVDTIDE AMDHIARWGS GHTDAIVTEN LSAAQRFQRE 370 380 390 400 410 VDSSSVYVNL PTCFADGFEY GLGAEIGIST NRLHARGPVG LEGLTTLKWV LNGEGQVRG

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640428 Genomic DNA. Translation: CAE37029.1.
RefSeq	NP_884003.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1667047.
GenomeReviews	Gene locus BPP1728 in contig BX470249_GR.
KEGG	bpa:BPP1728.
NMPDR	fig|257311.1.peg.1637.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7W9M7.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP1728-MON.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BORPA

Accession

Primary (citable) accession number: Q7W9M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)