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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei642 – 6421ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPAR257311:BPP1731-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BPP1731
OrganismiBordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)
Taxonomic identifieri257311 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
ProteomesiUP000001421 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 885885Leucine--tRNA ligasePRO_0000151981Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi257311.BPP1731.

Structurei

3D structure databases

ProteinModelPortaliQ7W9M4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi639 – 6435"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7W9M4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQERYQPNSV EAAAQQTWQA RDAYLVHEHA KNPDGSEKPK FYACSMLPYP
60 70 80 90 100
SGKLHMGHVR NYTINDMMAR QLRMRGYNVL MPMGWDAFGM PAENAAIKSK
110 120 130 140 150
VPPAKWTYDN IAYMKKQMKA MGLAIDWSRE MCACDPKYYK WNQWLFLKML
160 170 180 190 200
EKGIAYRKTQ VVNWDPVDQT VLANEQVIDG RGWRSGAPVE KREIPGYYLR
210 220 230 240 250
ITDYAEELLD QVSTNLPGWP ERVRLMQENW IGKSEGLRFA FPHRIAGADG
260 270 280 290 300
KLIQDGKLYV FTTRADTIMG VTFCAVAPEH PLATHAAQSN PALAAFVEQC
310 320 330 340 350
KLGGTTEAEM ATREKEGMPT GLTVTHPLTG AEIDVWVGNY VLMTYGDGAV
360 370 380 390 400
MGVPAHDERD FAFARKYGLP IRQVVALEGK TYSTDAWQEW YGDKQAGRTV
410 420 430 440 450
NSGKYDGLAY QAAVDTIAAD LAAQGLGEKQ TTWRLRDWGI SRQRYWGTPI
460 470 480 490 500
PIIHCADCGP VPVPEQDLPV VLPDDLIPDG SGNPLAKNEA FLSCSCPRCG
510 520 530 540 550
KPARRETDTM DTFVDSSWYF MRYTSPDNDQ AMVDARNDYW MPMDQYIGGI
560 570 580 590 600
EHAVLHLLYA RFWTKVMRDL SLLNFDEPFT KLLCQGMVLN HIYSRKTPQG
610 620 630 640 650
GIEYFWPEEV DNVYDAKGAI VGAKLQRDGS EVNYGGVGTM SKSKNNGVDP
660 670 680 690 700
QSLIDTLGAD TARLFVMFAS PPEQTLEWSD SGVEGANRFL RRLWALGYAQ
710 720 730 740 750
REAVGRGLAT GADWAQAPAP VKELRREVYG LLKQADYDYQ RIQYNTVVSA
760 770 780 790 800
CMKMLNAIDD APLPEGPAAD AARAETLGLL LRVLYPVVPH ITWHLWQDLG
810 820 830 840 850
YAEHLGDLLD APWPHVDEAA LVADEIELML QVNGKLRGSI RVAAKAPKED
860 870 880
IERIAAAQEE VARFLEGRPP KRVIVVPGKL VNVVG
Length:885
Mass (Da):99,038
Last modified:October 1, 2003 - v1
Checksum:i7E5447E327A21C52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640428 Genomic DNA. Translation: CAE37032.1.
RefSeqiNP_884006.1. NC_002928.3.
WP_010928174.1. NC_002928.3.

Genome annotation databases

KEGGibpa:BPP1731.
PATRICi21147227. VBIBorPar43418_1816.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640428 Genomic DNA. Translation: CAE37032.1.
RefSeqiNP_884006.1. NC_002928.3.
WP_010928174.1. NC_002928.3.

3D structure databases

ProteinModelPortaliQ7W9M4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257311.BPP1731.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGibpa:BPP1731.
PATRICi21147227. VBIBorPar43418_1816.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBPAR257311:BPP1731-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica."
    Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S.
    , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J.
    Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Entry informationi

Entry nameiSYL_BORPA
AccessioniPrimary (citable) accession number: Q7W9M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2003
Last modified: April 1, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.