Q7W912 (SYT_BORPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BPP1963

Organism

Bordetella parapertussis [Complete proteome] [HAMAP]

Taxonomic identifier

519 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000020348
Regions
	Region	247 – 538	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	338	1	Zinc; catalytic By similarity
	Metal binding	389	1	Zinc; catalytic By similarity
	Metal binding	515	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7W912 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D67226AFDF415101	FASTA	649	73,324
	10 20 30 40 50 60 MVQITLPDGS QRQYPGPVTV AEVAQSIGAG LAKAALAGRV AFDGAEPRLV DTSFRIDNDA 70 80 90 100 110 120 QLAIVTAKDA DGLDLIRHST AHLLAYAVKS LFPDAQVTIG PVIDNGFYYD FSYKRPFTPE 130 140 150 160 170 180 DLQAIEKKMA ELARKDEVVT REEWSRDEAV AYFKGIGEVY KAEIIASIPS NETLSLYREG 190 200 210 220 230 240 DFIDLCRGPH VPSTGKLKVF KLMKVAGAYW RGDSKNEMLQ RIYGTAWASK DDQDAYLHML 250 260 270 280 290 300 EEAERRDHRK IGRELDLFHF QDEAPGLIFW HPKGWALWQQ VEQYMRKVYQ DNGYQEVKAP 310 320 330 340 350 360 QILDLTLWKK TGHWDNYREN MFTTESENRV YGLKPMNCPG HVQIFNAGLH SYRELPLRYG 370 380 390 400 410 420 EFGQCHRNEP SGSLHGMMRV RGFTQDDGHI FCTEDQLQDE CAAFTALLQK VYKDFGFTEV 430 440 450 460 470 480 LYKVATRPEK RIGSDEIWDK AETALMESLR RTGCEFEISP GEGAFYGPKV EYTLKDAIGR 490 500 510 520 530 540 HWQCGTIQVD FSMPVRLGAE YVDQNDQRRP PVMLHRAILG SLERFIGMLI ENHAGAMPPW 550 560 570 580 590 600 LAPLQAVVCC ISEHSAEYAA QITQSLKKQG FRVQADLRGE KITRKIREHS LQKIPYLLVV 610 620 630 640 GDKEMQNGTV AVRGLGGLDL GVIALDDFIA RLAEDISTRR NVTQLASAA

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640429 Genomic DNA. Translation: CAE37262.1.
RefSeq	NP_884223.1. NC_002928.3.
3D structure databases
HSSP	HSSP built from PDB template 1EVL based on UniProtKB P0A8M3.
ProteinModelPortal	Q7W912.
SMR	Q7W912. Positions 246-640.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1667920.
GenomeReviews	Gene locus BPP1963 in contig BX470249_GR.
KEGG	bpa:BPP1963.
PATRIC	21147740. VBIBorPar43418_2068.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352811.
OMA	MIIRNIL.
PhylomeDB	Q7W912.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP1963-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BORPA

Accession

Primary (citable) accession number: Q7W912

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program