Q7W910 (GSHB_BORPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione synthetase
EC=6.3.2.3

Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase

Gene names

Name:	gshB
Synonyms:	gsh-II
Ordered Locus Names:	BPP1965

Organism

Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253) [Complete proteome] [HAMAP]

Taxonomic identifier

257311 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella › Bordetella parapertussis

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Glutathione synthetase HAMAP-Rule MF_00162		PRO_0000197454
Regions
	Domain	129 – 314	186	ATP-grasp
	Nucleotide binding	155 – 211	57	ATP By similarity
Sites
	Metal binding	285	1	Magnesium or manganese By similarity
	Metal binding	287	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7W910 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 5902082AD5DC4F97	FASTA	318	34,652
	10 20 30 40 50 60 MHVLFIIDPL PLLKAYKDSS VAMMQALQAR GHTLSVALQG DLYIDAGEVR TRFAPIALRD 70 80 90 100 110 120 GADLHGHDWW RETGAADEAP LARFDAVVMR KDPPFDMEYV YSTHLLEYAQ QQGARVFNSG 130 140 150 160 170 180 AAIRNHPEKL AITEFPDLTT PTLVTRDMGR IRAFHAAQGD VIVKPLDGMG GTGIFRLQRS 190 200 210 220 230 240 EPNLNAILET LTDNGTRTIM AQRYIPEIVK GDKRILLIGG EPVPYSLARI PLAGETRGNL 250 260 270 280 290 300 AAGGRGVAQP LSERDLHLAR TVADRLAGRG LLLVGLDVIG DYITEVNVTS PTCFVEITEQ 310 TGFNVPEMFA VALESAAG

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640429 Genomic DNA. Translation: CAE37264.1.
RefSeq	NP_884225.1. NC_002928.3.
3D structure databases
ProteinModelPortal	Q7W910.
ModBase	Search...
Protein-protein interaction databases
STRING	257311.BPP1965.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE37264; CAE37264; BPP1965.
GeneID	1666938.
KEGG	bpa:BPP1965.
PATRIC	21147744. VBIBorPar43418_2070.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0189.
HOGENOM	HOG000265022.
KO	K01920.
OMA	WMRKDPP.
ProtClustDB	PRK05246.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP1965-MONOMER.
UniPathway	UPA00142; UER00210.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit.
HAMAP	MF_00162. GSH-S.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP-bd. IPR004215. GSHS_N. IPR016185. PreATP-grasp_dom. [Graphical view]
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_BORPA

Accession

Primary (citable) accession number: Q7W910

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program