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Q7W8Z2

- SYI_BORPA

UniProt

Q7W8Z2 - SYI_BORPA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei582 – 5821Aminoacyl-adenylateUniRule annotation
    Binding sitei626 – 6261ATPUniRule annotation
    Metal bindingi916 – 9161ZincUniRule annotation
    Metal bindingi919 – 9191ZincUniRule annotation
    Metal bindingi936 – 9361ZincUniRule annotation
    Metal bindingi939 – 9391ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBPAR257311:BPP1984-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BPP1984
    OrganismiBordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)
    Taxonomic identifieri257311 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
    ProteomesiUP000001421: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Isoleucine--tRNA ligasePRO_0000098358Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi257311.BPP1984.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7W8Z2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi623 – 6275"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7W8Z2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKKSLNLP DTPFPMRGDL AKREPGWVAE WEETQVYQAI RAASRGRPRF    50
    VLHDGPPYAN GDIHIGHAVN KILKDIIVKS RNMAGYDAHY VPGWDCHGMP 100
    IEIQIEKKYG KHLPVTEVQS KARAYALEQI DRQRKDFKRL GVLGDWHNPY 150
    LTMNFSNEAD EIRVLGRILE KGYVFRGLKP VNWCFDCGSA LAEAEVEYAD 200
    RVDPAIDVAF PFTDRGALAR AFGLDEVDAG AIVIWTTTPW TIPSNQALNV 250
    HPEIDYALVR VTPTPVHGPL LLLAQERVEP SLKAWGLEGE IIATAKGEAL 300
    EGLRFRHPLA AAAQGYDRTS PIYLGDYVTL DTGTGVVHSA PAYGIEDFVS 350
    CKAHGLADSD ILGPVMGDGK FVDSLPLFGG LSIWDANPRI VEALKLAGSL 400
    MLVQKLSHSY MHCWRHKTPV IYRATSQWFA GMDVKPRDGG PSLRESALAG 450
    IDATAFYPAW GRARLHAMIA NRPDWTLSRQ RQWGVPMAFF VHKETGELHP 500
    RTVELLEQVA QRVEKGGIEA WQSLDPRELL GDEAELYEKN RDTLDVWFDS 550
    GSTHATVLGG KDGVLGSSHG AELAWPADLY LEGSDQHRGW FHSSLLTGCM 600
    LYGHPPYKGL LTHGFVVDGQ GRKMSKSVGN VIAPQKVSDS LGAEILRLWV 650
    ASTDYSGELS ISDEILKRVV ESYRRIRNTL RFLLANVADF DAVGQAVPYG 700
    ELFEIDRYAL AMTAQMQAEV QGHYERYDFH PAVSRLQTFC SEDLGAFYLD 750
    ILKDRLYTTA AGSAARRSAQ TALLDITQTL LKLMAPILSF TAEEAWKVLA 800
    GSALAKQADA PRVTIFTEVY HALPPFADGE ALTAKWTRLR AIRAEVQRKL 850
    EEVRSAGAIG SSLQAEVDLY ANAADHDILA SLGDDLRFVL IVSRATVHAD 900
    ADDLRIEIAA SGHKKCERCW HWRPDVGQDA DHPEICGRCV SNLFGAGEPR 950
    TRA 953
    Length:953
    Mass (Da):105,561
    Last modified:October 1, 2003 - v1
    Checksum:i3E1887759A135559
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX640429 Genomic DNA. Translation: CAE37284.1.
    RefSeqiNP_884243.1. NC_002928.3.
    WP_010928306.1. NC_002928.3.

    Genome annotation databases

    EnsemblBacteriaiCAE37284; CAE37284; BPP1984.
    GeneIDi1664819.
    KEGGibpa:BPP1984.
    PATRICi21147785. VBIBorPar43418_2090.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX640429 Genomic DNA. Translation: CAE37284.1 .
    RefSeqi NP_884243.1. NC_002928.3.
    WP_010928306.1. NC_002928.3.

    3D structure databases

    ProteinModelPortali Q7W8Z2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257311.BPP1984.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE37284 ; CAE37284 ; BPP1984 .
    GeneIDi 1664819.
    KEGGi bpa:BPP1984.
    PATRICi 21147785. VBIBorPar43418_2090.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BPAR257311:BPP1984-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica."
      Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S.
      , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J.
      Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 12822 / ATCC BAA-587 / NCTC 13253.

    Entry informationi

    Entry nameiSYI_BORPA
    AccessioniPrimary (citable) accession number: Q7W8Z2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3