Phosphonoacetaldehyde hydrolase - Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phosphonoacetaldehyde hydrolase
Short name=Phosphonatase
EC=3.11.1.1

Alternative name(s):
Phosphonoacetaldehyde phosphonohydrolase

Gene names

Name:	phnX
Ordered Locus Names:	BPP2453

Organism

Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253) [Complete proteome] [HAMAP]

Taxonomic identifier

257311 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella ›

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in phosphonate degradation By similarity.
Catalytic activity	Phosphonoacetaldehyde + H₂O = acetaldehyde + phosphate. HAMAP-Rule MF_01375
Cofactor	Binds 1 Mg²⁺ ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PhnX family.
Caution	The first enzyme involved in phosphonate degradation (PhnW, EC 2.6.1.37) is not found in this organism. The function of this enzyme is therefore uncertain.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Schiff base
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	organic phosphonate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphonoacetaldehyde hydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 276

276

Phosphonoacetaldehyde hydrolase HAMAP-Rule MF_01375

PRO_0000284585

Sites

Active site

19

1

Nucleophile By similarity

Active site

60

1

Schiff-base intermediate with substrate By similarity

Metal binding

19

1

Magnesium By similarity

Metal binding

21

1

Magnesium; via carbonyl oxygen By similarity

Metal binding

193

1

Magnesium By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q7W7R1 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 09127118A5C48638
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FASTA

276

29,871

        10         20         30         40         50         60 
MNAMTLATLP VRLEALVFDW AGTLVDFGSF APTKVFVDAF ARFGVQISLE QARGPMGMGK 

        70         80         90        100        110        120 
WDHIRALCND AAIARQYQEQ FGRLPTDEDV TAIYERFLPM QLEKVAEYSQ PIPGAIELLH 

       130        140        150        160        170        180 
GLRQRGLKLG SCSGYPAAVM QRVLERAERE GLALDYVVAS DDVPRSRPAP AMALRNVVEL 

       190        200        210        220        230        240 
GIADVAGCVK VDDTAPGVEE GRRAGMWTVG LLLSGNAAGL SLEQFLSLDE AGREAARDRA 

       250        260        270 
RAELQAGAPH YLIDTVADLP PVLADIETRL AAGQRP

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References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica."
Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S.

Maskell D.J.
Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640430 Genomic DNA. Translation: CAE37748.1.
RefSeq	NP_884684.1. NC_002928.3.
3D structure databases
ProteinModelPortal	Q7W7R1.
ModBase	Search...
Protein-protein interaction databases
STRING	257311.BPP2453.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE37748; CAE37748; BPP2453.
GeneID	1668176.
KEGG	bpa:BPP2453.
PATRIC	21148783. VBIBorPar43418_2576.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0637.
HOGENOM	HOG000217971.
KO	K05306.
OMA	EFGATWE.
ProtClustDB	PRK13478.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP2453-MONOMER.
Family and domain databases
Gene3D	1.10.150.240. 1 hit. 3.40.50.1000. 2 hits.
HAMAP	MF_01375. PhnX.
InterPro	IPR023214. HAD-like_dom. IPR006402. HAD-SF_hydro_IA_v3. IPR023198. PGP_dom2. IPR006323. Phosphonoacetald_hydro. [Graphical view]
Pfam	PF13419. HAD_2. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01422. phosphonatase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHNX_BORPA

Accession

Primary (citable) accession number: Q7W7R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents