Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q7W733 (NAPA_BORPA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:BPP2702
OrganismBordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253) [Complete proteome] [HAMAP]
Taxonomic identifier257311 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella

Protein attributes

Sequence length831 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3838Tat-type signal Potential
Chain39 – 831793Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045978

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7W733 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 7A9ED7B93B241585

FASTA83193,168
        10         20         30         40         50         60 
MSMARRDFIK QTAAAAAATV AGVPLTGYTQ NIVTESEAAK LKWSKAPCRF CGTGCGVNVA 

        70         80         90        100        110        120 
VKDNQVVATH GDFNAEVNKG LNCVKGYFLS KIMYGSDRLT QPLLRIKDGK YAKDGEFAPV 

       130        140        150        160        170        180 
SWDQAFDVMA EQFKRVLKDK GPEAVGMFGS GQWTVWEGYA ALKLMKAGFR TNNLDPNARH 

       190        200        210        220        230        240 
CMASAAVGFM RTFGADEPMG CYDDIENADA FVLWGSNMAE MHPILWTRVT DRRLSAPATK 

       250        260        270        280        290        300 
VAVLSTFEHR SYELADLTLT FEPQSDLAIL NYIANHIIRT KRVNRDFVDK HTVFREGNAD 

       310        320        330        340        350        360 
IGYGLRPEHP LQQAARNAGD AGGSKPITFD DFARFVSKYD LEYTAKLSGV PKNRLEELAE 

       370        380        390        400        410        420 
LYADPKVRVT SFWTMGFNQH TRGVWCNNMV YNIHLLTGKI STPGNSPFSL TGQPSACGTA 

       430        440        450        460        470        480 
REVGTFSHRL PADLVVTNPE HRRHAEEIWK LPDGTIPSKV GAHAVLQNRM LKDGKINAYW 

       490        500        510        520        530        540 
VMVNNNMQAA ANLMNEGLPG YRNPENFIVV SDAYPTVTTL SADLILPAAM WVEKEGAYGN 

       550        560        570        580        590        600 
AERRTQFWHQ LVDAPGQARS DLWQLVEFSK RFKVEEVWPA DLLAKKPEYR GKTLYDVLFA 

       610        620        630        640        650        660 
NGKVNQFPNT ELDPEYANQE AQAFGFYLQK GLFEEYAEFG RGHGHDLAPF DVYHKARGLR 

       670        680        690        700        710        720 
WPVVDGKETL WRYREGSDPY VKPGTGFQFY GNPDGKAVIF ALPYEPPPEA PDKEYPFWLS 

       730        740        750        760        770        780 
TGRVLEHWHS GSMTRRVPEL YKAFPEAVCF MHPDDAQALG VRRGVEVEVV SRRGKMRTRV 

       790        800        810        820        830 
ETRGRDKPPR GLVFVPWFDA GQLINKVTLD ATDPISFQTD FKKCAVKIVK V 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX640431 Genomic DNA. Translation: CAE37995.1.
RefSeqNP_884918.1. NC_002928.3.

3D structure databases

ProteinModelPortalQ7W733.
SMRQ7W733. Positions 41-828.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257311.BPP2702.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE37995; CAE37995; BPP2702.
GeneID1665471.
KEGGbpa:BPP2702.
PATRIC21149320. VBIBorPar43418_2838.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMARRTHVWH.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycBPAR257311:BPP2702-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_BORPA
AccessionPrimary (citable) accession number: Q7W733
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2003
Last modified: February 19, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families