Q7W5K6 (AMPA_BORPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BPP3285

Organism

Bordetella parapertussis [Complete proteome] [HAMAP]

Taxonomic identifier

519 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165725
Sites
	Active site	283	1	Potential
	Active site	357	1	Potential
	Metal binding	271	1	Manganese 2 By similarity
	Metal binding	276	1	Manganese 1 By similarity
	Metal binding	276	1	Manganese 2 By similarity
	Metal binding	294	1	Manganese 2 By similarity
	Metal binding	353	1	Manganese 1 By similarity
	Metal binding	355	1	Manganese 1 By similarity
	Metal binding	355	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7W5K6 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 72A7E425EDB97D71	FASTA	499	51,729
	10 20 30 40 50 60 MEFSTQTTAS LHQIKTAALA VGVFADGVLS AAAEVIDRAS HGAVAAVVKS EFRGRTGGTL 70 80 90 100 110 120 VLRSLAGVSA QRVVLVGLGK QAEYNARAHA SAEQAFAAAC VAAQVGEGVS TLAGVAIEGV 130 140 150 160 170 180 PVRARARSAA IAAGAAAYHY DATFGKANRD ARPRLKKIVQ VVDRAASAQT QLGLREGAAI 190 200 210 220 230 240 AHGMELTRTL GNLPGNVCTP AYLGNTAKKL AREFKSLKVE VLERKQVEAL GMGSFLSVAR 250 260 270 280 290 300 GSEEPLRFIV LRHAGKPAKK DKAGPVVLVG KGITFDAGGI SLKPAATMDE MKYDMCGAAS 310 320 330 340 350 360 VLGTFRALAE LELPLDVVGL IAACENLPSG KANKPGDVVT SMSGQTIEIL NTDAEGRLVL 370 380 390 400 410 420 CDALTYAERF KPAAVIDIAT LTGACVVALG NVNSGLFSKD DALADALLAA SRQSLDPAWR 430 440 450 460 470 480 LPLDDAYQDQ LKSNFADIAN IGGPPAGAVT AACFLSRFTK AYPWAHLDIA GTAWRGGKDK 490 GATGRPVPLL MQYLLDQAG

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640433 Genomic DNA. Translation: CAE38570.1.
RefSeq	NP_885452.1. NC_002928.3.
3D structure databases
ProteinModelPortal	Q7W5K6.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1664754.
GenomeReviews	Gene locus BPP3285 in contig BX470249_GR.
KEGG	bpa:BPP3285.
NMPDR	fig|257311.1.peg.3086.
PATRIC	21150550. VBIBorPar43418_3450.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742580.
OMA	KYDWAHL.
PhylomeDB	Q7W5K6.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP3285-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BORPA

Accession

Primary (citable) accession number: Q7W5K6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program