Reviewed, UniProtKB/Swiss-Prot Q7W515 (HEMH_BORPA)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	BPP3489

Organism

Bordetella parapertussis [Complete proteome] [HAMAP]

Taxonomic identifier

519 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Ferrochelatase HAMAP MF_00323		PRO_0000175117
Sites
	Metal binding	228	1	Iron By similarity
	Metal binding	309	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7W515-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 8B1A488E71215984	FASTA	362	40,902
	10 20 30 40 50 60 MFLRLFKYLW PERYLPEIPA QDPFDENPPP CGPGRVGVLL VNLGTPDEPT RGAIRRYLGE 70 80 90 100 110 120 FLSDPRVIEI PRYLWMPILH GLVLAMRPKK LAPRYAGIWM EEGSPLLVYS QRQAAGVRQG 130 140 150 160 170 180 LAARGVHAEV ELAMRYGKPS IPAAITALRE RGCDHILAVP LYPQYAASTT ATVVDAVTRH 190 200 210 220 230 240 AGRLRDQPAL RFVKRFHNDP AYVEAQAGRI AEFWQAHGRP QKLVMSFHGL PRYSIELGDP 250 260 270 280 290 300 YYRDCLDTAR LLRERLGLRE DEVEVTFQSR FGSARWLEPY TEPTLAELAR QGVTEVDVVC 310 320 330 340 350 360 PGFVADCLET LEEISQECRD AFVAAGGRQF RYIPALNDCP PWIEGLTDLV ERQLRGWPTG NP

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640433 Genomic DNA. Translation: CAE38773.1.
RefSeq	NP_885649.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1664633.
GenomeReviews	Gene locus BPP3489 in contig BX470249_GR.
KEGG	bpa:BPP3489.
NMPDR	fig|257311.1.peg.3283.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7W515.
OMA	MSFHGLP.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP3489-MON.
BRENDA	4.99.1.1. 263123.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BORPA

Accession

Primary (citable) accession number: Q7W515

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)