Q7W497 (ILVD2_BORPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 2
Short name=DAD 2
EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	BPP3770

Organism

Bordetella parapertussis [Complete proteome] [HAMAP]

Taxonomic identifier

519 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.
Sequence caution	The sequence CAE39053.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000103438
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7W497 [UniParc]. Last modified December 15, 2003. Version 2. Checksum: 3CF8F20CB5005C9E	FASTA	561	59,718
	10 20 30 40 50 60 MSDNNRSRHI TEGVARAPNR AMYYALGYTE ADFQNPMIGV ANGHSTITPC NSGLQRLADA 70 80 90 100 110 120 AIEAIRVSRA NPQVFGTPTI SDGMSMGTEG MKYSLVLREV IADCIETAAQ GQWMDGVVVI 130 140 150 160 170 180 GGCDKNMPGG MMALARMNVP GIYVYGGTIK PGHYKGKDLT IVSVFEAVGE YTMGRMDETD 190 200 210 220 230 240 FKAIEQCAIP GSGSCGGMYT ANTMSSAFEA MGMSLPHSST MANEDQEKVA SAAESARVLV 250 260 270 280 290 300 EAVRRQLRPR DIITRASIEN AVAVIMATGG STNAVLHFLA IAHAAEVPWN IDDFERIRKR 310 320 330 340 350 360 VPVICDLKPS GKYVATDLHR AGGIPQVMKI LLNAGLLHGD CITITGKTVA ETLANVPDAP 370 380 390 400 410 420 PPGQDVIMPI ERAFYPQGHL AILKGNLSPE GCVAKITGLK NPVITGPARV FDSEDDAMSA 430 440 450 460 470 480 IMDRRIRDGD VVVIRYEGPK GGPGMREMLA PTSALVGQGL GETVGLITDG RFSGGTWGMV 490 500 510 520 530 540 VGHVAPEAFV GGPIALIREG DSVTIDAHQL LLQLNISDEE MAARRKAWAQ PKPRYVRGVL 550 560 AKFGKLACTA SRGAVTDAFE E

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640434 Genomic DNA. Translation: CAE39053.1. Different initiation.
RefSeq	NP_885923.2. NC_002928.3.
3D structure databases
ProteinModelPortal	Q7W497.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1668225.
GenomeReviews	Gene locus BPP3770 in contig BX470249_GR.
KEGG	bpa:BPP3770.
NMPDR	fig|257311.1.peg.3557.
PATRIC	21151591. VBIBorPar43418_3956.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
PhylomeDB	Q7W497.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP3770-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_BORPA

Accession

Primary (citable) accession number: Q7W497

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program