Reviewed, UniProtKB/Swiss-Prot Q7W3I6 (ARGB_BORPA)

Names and origin

Protein names

Recommended name:
    Acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name=AGK
    N-acetyl-L-glutamate 5-phosphotransferase

Gene names

Name:	argB
Ordered Locus Names:	BPP4047

Organism

Bordetella parapertussis [Complete proteome] [HAMAP]

Taxonomic identifier

519 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082
Subcellular location	Cytoplasm Probable HAMAP MF_00082.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Acetylglutamate kinase HAMAP MF_00082		PRO_0000112593
Regions
	Region	73 – 74	2	Substrate binding By similarity
Sites
	Binding site	95	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Site	38	1	Transition state stabilizer By similarity
	Site	257	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7W3I6-1 [UniParc]. Last modified December 15, 2003. Version 2. Checksum: 78A7B400B6C640C2	FASTA	300	31,771
	10 20 30 40 50 60 MTDTPDPAAV LSPAVKAAVL SEALPYIRRF HGKTIVVKYG GNAMTEERLQ RSFAHDVVLL 70 80 90 100 110 120 KLVGLNPVVV HGGGPQIDDA LRRIGKQGTF VQGIRVTDAE TMEVVEWVLG GQVQQDIVMM 130 140 150 160 170 180 INEVGGKAVG LTGKDGMLIQ ATKKLMVNKD DPSQPLDIGF VGDITRVEPA VVKALQDDQF 190 200 210 220 230 240 IPVISPIGYG EDGTAYNINA DVVAGKMAEV LGAEKLLMMT NTPGVLDKGG KLLRSLSAQT 250 260 270 280 290 300 IDELFADGTI SGGMLPKISS SLDAAKNGVN SVHIVDGRVP HCLLLEILTD QGVGTMISSH

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640435 Genomic DNA. Translation: CAE39330.1. Different initiation.
RefSeq	NP_886192.2.
3D structure databases
SMR	Q7W3I6. Positions 17-299.
ModBase	Search...
Genome annotation databases
GeneID	1665713.
KEGG	bpa:BPP4047.
NMPDR	fig|257311.1.peg.3826.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497643.
PhylomeDB	Q7W3I6.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP4047-MONOMER.
BRENDA	2.7.2.8. 263123.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR011148. GlcNAc_kinase. IPR001057. Glu_5kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BORPA

Accession

Primary (citable) accession number: Q7W3I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)