Reviewed, UniProtKB/Swiss-Prot Q7W2P4 (ACEK_BORPA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	BPP4360

Organism

Bordetella parapertussis [Complete proteome] [HAMAP]

Taxonomic identifier

519 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000057895
Regions
	Nucleotide binding	335 – 341	7	ATP By similarity
Sites
	Active site	390	1	By similarity
	Binding site	356	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7W2P4-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: A4F10F6987971226	FASTA	600	68,516
	10 20 30 40 50 60 MIYSGDVQRI EPAPVAGPAP LDVAHLILAG FDRHYALFRY SAQRAKSLFE SGDWHGMQRL 70 80 90 100 110 120 SRERIEYYDM RVRECATQLD SALRGSDART ADGSRANGSA ALSEAQTAFW QAVKQEFVGL 130 140 150 160 170 180 LADHRQPECA ETFFNSVSCR ILHRDYFHND FLFVRPAIAT DYLDSRIPSY RVYYPVAEGL 190 200 210 220 230 240 HKSLIRMVAD FGLAVPYADL PRDAGPRLAS DCQIQVLGSL FFRNTGAYIV GRLINQGTVY 250 260 270 280 290 300 PFAVALRRNP AGQVCLDALL LGTDDLSTLF SFTRAYFLVD METPAAVVNF LASLLPRKPK 310 320 330 340 350 360 AELYTMLGLQ KQGKTLFYRD FLHHLTHSRD AFDIAPGIRG MVMCVFTLPS YPYVFKLIKD 370 380 390 400 410 420 RIDKDGMDHA TVRRKYQMVK LHDRVGRMAD TWEYSQVALP RSRFAPRLLE ELRRLVPSLI 430 440 450 460 470 480 EENGDTVVIR HVYIERRMMP LNLYLRHASD PLLEVAVREY GDAIRQLATA NIFPGDMLYK 490 500 510 520 530 540 NFGVTRLGRV VFYDYDEIQR MTEMNFRAIP PAPNEEAELS SEPWYAVGPN DVFPEEFGRF 550 560 570 580 590 600 LLGDPRVRQA FLRHHADLLA PQWWQACRAR VAQGRIEEFF PYDTDRRLHP QAAPPPRAAA

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640436 Genomic DNA. Translation: CAE39639.1.
RefSeq	NP_886488.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1665567.
GenomeReviews	Gene locus BPP4360 in contig BX470249_GR.
KEGG	bpa:BPP4360.
NMPDR	fig|257311.1.peg.4122.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7W2P4.
OMA	FYRDFLH.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP4360-MON.
BRENDA	2.7.11.5. 263123.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_BORPA

Accession

Primary (citable) accession number: Q7W2P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)