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Protein

Malate synthase G

Gene

glcB

Organism
Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei275Acetyl-CoAUniRule annotation1
Binding sitei312Acetyl-CoAUniRule annotation1
Active sitei339Proton acceptorUniRule annotation1
Binding sitei339GlyoxylateUniRule annotation1
Metal bindingi428MagnesiumUniRule annotation1
Binding sitei428GlyoxylateUniRule annotation1
Metal bindingi456MagnesiumUniRule annotation1
Binding sitei537Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei632Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:BPP0096
OrganismiBordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)
Taxonomic identifieri257311 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000001421 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000568941 – 725Malate synthase GAdd BLAST725

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei618Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ7W294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni453 – 456Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7W294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERIPHHGL QVAASLHRFI EDEALSGSGL APDEFWAGFA ALVRDLAPRN
60 70 80 90 100
RELLAERDRL QGEIDAWHRA HPGPVRDSAG YQALLERIGY LQPQPAQVTA
110 120 130 140 150
STRDVDSEIA SQAGPQLVVP VSNARYALNA ANARWGSLYD ALYGTDAIPP
160 170 180 190 200
VAGDDGKGYN PARGEAVIAR ARAFLDEAAP LAQGSHADAT AYAIEGGKLA
210 220 230 240 250
VTLGAGQRTG LRNPAQLAGY QGDASQPAAV LLANNGLHFE IQIDRQHQIG
260 270 280 290 300
ATDAAGVKDV LLEAALTTIM DCEDSVAAVD ADDKVLIYRN WLGLMKGDLS
310 320 330 340 350
ESVTKGGKTF TRRLNADRQY HKPDGGTLTL HGRSLMFVRN VGHLMTNPAI
360 370 380 390 400
LDEQGNEVPE GILDAVITSL AALPDRANRL NSRTGSIYIV KPKMHGPAEA
410 420 430 440 450
AFANELFDRV EDLLKLPRHT IKMGIMDEER RTSVNLKACI AAAAARVAFI
460 470 480 490 500
NTGFLDRTGD EMHTGMEAGP MLRKGDMKSS AWITAYERNN VLVGLDCGLR
510 520 530 540 550
GRAQIGKGMW AMPDMMAAML EQKIGHPKAG ANTAWVPSPT AATLHAMHYH
560 570 580 590 600
QVDVAAVQQA LEQTRYDSVR DELLAGLLTV PVGDPAAWSA DDIQRELDNN
610 620 630 640 650
AQGILGYVVR WIDQGVGCSK VPDINNVGLM EDRATLRISS QHIANWLRHG
660 670 680 690 700
IVDRAQVNAT FERMAKVVDQ QNAGDPNYLP MAGHFDTSFA YRAACALVFE
710 720
GLTQPNGYTE PLLHEYRQAF KAAQR
Length:725
Mass (Da):78,547
Last modified:October 1, 2003 - v1
Checksum:i56CFA5BAAD16127C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640423 Genomic DNA. Translation: CAE39837.1.
RefSeqiWP_010925701.1. NC_002928.3.

Genome annotation databases

EnsemblBacteriaiCAE39837; CAE39837; BPP0096.
KEGGibpa:BPP0096.
PATRICi21143751. VBIBorPar43418_0104.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640423 Genomic DNA. Translation: CAE39837.1.
RefSeqiWP_010925701.1. NC_002928.3.

3D structure databases

ProteinModelPortaliQ7W294.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE39837; CAE39837; BPP0096.
KEGGibpa:BPP0096.
PATRICi21143751. VBIBorPar43418_0104.

Phylogenomic databases

HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_BORPA
AccessioniPrimary (citable) accession number: Q7W294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2003
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.