Q7W294 (MASZ_BORPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	BPP0096

Organism

Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253) [Complete proteome] [HAMAP]

Taxonomic identifier

257311 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella › Bordetella parapertussis

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Malate synthase G HAMAP-Rule MF_00641		PRO_1000056894
Sites
	Active site	339	1	Proton acceptor By similarity
	Active site	632	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7W294 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 56CFA5BAAD16127C	FASTA	725	78,547
	10 20 30 40 50 60 MTERIPHHGL QVAASLHRFI EDEALSGSGL APDEFWAGFA ALVRDLAPRN RELLAERDRL 70 80 90 100 110 120 QGEIDAWHRA HPGPVRDSAG YQALLERIGY LQPQPAQVTA STRDVDSEIA SQAGPQLVVP 130 140 150 160 170 180 VSNARYALNA ANARWGSLYD ALYGTDAIPP VAGDDGKGYN PARGEAVIAR ARAFLDEAAP 190 200 210 220 230 240 LAQGSHADAT AYAIEGGKLA VTLGAGQRTG LRNPAQLAGY QGDASQPAAV LLANNGLHFE 250 260 270 280 290 300 IQIDRQHQIG ATDAAGVKDV LLEAALTTIM DCEDSVAAVD ADDKVLIYRN WLGLMKGDLS 310 320 330 340 350 360 ESVTKGGKTF TRRLNADRQY HKPDGGTLTL HGRSLMFVRN VGHLMTNPAI LDEQGNEVPE 370 380 390 400 410 420 GILDAVITSL AALPDRANRL NSRTGSIYIV KPKMHGPAEA AFANELFDRV EDLLKLPRHT 430 440 450 460 470 480 IKMGIMDEER RTSVNLKACI AAAAARVAFI NTGFLDRTGD EMHTGMEAGP MLRKGDMKSS 490 500 510 520 530 540 AWITAYERNN VLVGLDCGLR GRAQIGKGMW AMPDMMAAML EQKIGHPKAG ANTAWVPSPT 550 560 570 580 590 600 AATLHAMHYH QVDVAAVQQA LEQTRYDSVR DELLAGLLTV PVGDPAAWSA DDIQRELDNN 610 620 630 640 650 660 AQGILGYVVR WIDQGVGCSK VPDINNVGLM EDRATLRISS QHIANWLRHG IVDRAQVNAT 670 680 690 700 710 720 FERMAKVVDQ QNAGDPNYLP MAGHFDTSFA YRAACALVFE GLTQPNGYTE PLLHEYRQAF KAAQR

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640423 Genomic DNA. Translation: CAE39837.1.
RefSeq	NP_882459.1. NC_002928.3.
3D structure databases
HSSP	HSSP built from PDB template 1N8I based on UniProtKB P0A5J4.
ProteinModelPortal	Q7W294.
SMR	Q7W294. Positions 10-717.
ModBase	Search...
Protein-protein interaction databases
STRING	257311.BPP0096.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE39837; CAE39837; BPP0096.
GeneID	1668785.
KEGG	bpa:BPP0096.
PATRIC	21143751. VBIBorPar43418_0104.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HOG000220740.
KO	K01638.
OMA	SQFIENE.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP0096-MONOMER.
UniPathway	UPA00703; UER00720.
Family and domain databases
Gene3D	2.170.170.11. 2 hits.
HAMAP	MF_00641. Malate_synth_G.
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_BORPA

Accession

Primary (citable) accession number: Q7W294

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program