Reviewed, UniProtKB/Swiss-Prot Q7W1T9 (ASPD1_BORPA)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase 1
    EC=1.4.1.21

Gene names

Name:	nadX1
Ordered Locus Names:	BPP0257

Organism

Bordetella parapertussis [Complete proteome] [HAMAP]

Taxonomic identifier

519 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Probable L-aspartate dehydrogenase 1 HAMAP MF_01265		PRO_0000144883
Sites
	Active site	211	1	By similarity
	Binding site	121	1	NAD; via amide nitrogen By similarity
	Binding site	181	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7W1T9-1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 185F7E1B0C222E44	FASTA	258	26,302
	10 20 30 40 50 60 MNTPLRVGIV GCGVLANAMA GHLARQPRPV EIVGCLVRDP GRARGALPCH GSWEALLAQR 70 80 90 100 110 120 PEVVVECAGQ AALAQYAQAI LAAGVDLVPA SVGALADDAL RGALLEAAAA AGARIRIPSG 130 140 150 160 170 180 AMVGIDGLAA ARHVGVAEVL YRGTMPPVAL QRYVSGPLPE RGLAFAGSAR EAVARFPKNA 190 200 210 220 230 240 NLTGTIALAG IGFDRTRVEM LIDPDATANV HELLARGEFG DFHARVSGLR ISESSPSSRI 250 VAGSLAQAAL GSGFLALS

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12822 / ATCC BAA-587 / NCTC 13253.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640423 Genomic DNA. Translation: CAE39998.1. Different initiation.
RefSeq	NP_882616.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1668964.
GenomeReviews	Gene locus BPP0257 in contig BX470249_GR.
KEGG	bpa:BPP0257.
NMPDR	fig|257311.1.peg.250.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7W1T9.
Enzyme and pathway databases
BioCyc	BPAR257311:BPP0257-MON.
BRENDA	1.4.1.21. 263123.
Family and domain databases
HAMAP	MF_01265. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ASPD1_BORPA

Accession

Primary (citable) accession number: Q7W1T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	June 16, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)