Reviewed, UniProtKB/Swiss-Prot Q7VZG4 (SERC_BORPE)

Names and origin

Protein names

Recommended name:
    Phosphoserine aminotransferase
    EC=2.6.1.52
Alternative name(s):
    Phosphohydroxythreonine aminotransferase
      Short name=PSAT

Gene names

Name:	serC
Ordered Locus Names:	BP0945

Organism

Bordetella pertussis [Complete proteome] [HAMAP]

Taxonomic identifier

520 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity.
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP MF_00160
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. HAMAP MF_00160 Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. HAMAP MF_00160
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Pyridoxine biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Phosphoserine aminotransferase HAMAP MF_00160		PRO_0000150157
Regions
	Region	254 – 255	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	43	1	L-glutamate By similarity
	Binding site	105	1	Pyridoxal phosphate By similarity
	Binding site	164	1	Pyridoxal phosphate By similarity
	Binding site	189	1	Pyridoxal phosphate By similarity
	Binding site	212	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	213	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VZG4-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: CD489BE01053EA57	FASTA	377	40,858
	10 20 30 40 50 60 MMARPWNFSA GPSALPEAVL QQAAAEMLDW HGSGMSVMEM SHRGKHFVQI CDEAEADLRE 70 80 90 100 110 120 LLGLPADYAV MFMQGGGLGE NAIVPMNLMG RRSTPAADFV VTGHWSTRSH KEAGRYGDAQ 130 140 150 160 170 180 IAASAAEATE IDGQAQSSWT WLPPVDTWRV RKDSAYLHLC SNETIGGVEF TEWPDPASLG 190 200 210 220 230 240 APDVPLVVDV SSHFLSRPLD IARAGLVFAG AQKNAGPAGV TVVIARRDLL GHALPICPSA 250 260 270 280 290 300 FDYANVAADH SRYNTPPTFA IYVMGLVFKW IKAHGGVRGM EEANRAKAEL LYGYLDGSAF 310 320 330 340 350 360 YRNPVQASVR SRMNVPFVLR DESLNDAFLQ GAEAAGLMQL KGHKSVGGMR ASIYNAMPLA 370 GVQALIDYLK EFERRHG

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tohama I / ATCC BAA-589 / NCTC 13251.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX640413 Genomic DNA. Translation: CAE41247.1.
RefSeq	NP_879746.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2664164.
GenomeReviews	Gene locus BP0945 in contig BX470248_GR.
KEGG	bpe:BP0945.
NMPDR	fig|257313.1.peg.825.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VZG4.
OMA	SMYNTPP.
Enzyme and pathway databases
BioCyc	BPER257313:BP0945-MON.
BRENDA	2.6.1.52. 21511.
Family and domain databases
HAMAP	MF_00160. [Tree]
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR003248. Pser_amintransf. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
ProDom	PD001544. Pser_amintransf. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01364. serC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_BORPE

Accession

Primary (citable) accession number: Q7VZG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)