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Q7VY73 (LEU3_BORPE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydrogenase
EC=1.1.1.85
Alternative name(s):
3-IPM-DH
Beta-IPM dehydrogenase
Short name=IMDH
Gene names
Name:leuB
Ordered Locus Names:BP1483
OrganismBordetella pertussis
Taxonomic identifier520 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033

Subunit structure

Homodimer By similarity. HAMAP MF_01033

Subcellular location

Cytoplasm By similarity HAMAP MF_01033.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3583583-isopropylmalate dehydrogenase HAMAP MF_01033
PRO_0000083651

Regions

Nucleotide binding282 – 29413NAD By similarity

Sites

Metal binding2241Magnesium or manganese By similarity
Metal binding2481Magnesium or manganese By similarity
Metal binding2521Magnesium or manganese By similarity
Binding site921Substrate By similarity
Binding site1021Substrate By similarity
Binding site1301Substrate By similarity
Binding site2241Substrate By similarity
Site1371Important for catalysis By similarity
Site1921Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VY73 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 145DB5DBB951CEED

FASTA35838,557
        10         20         30         40         50         60 
MTHQIAVLPG DGIGPEIVEQ AERVLKALDL PLELRQAPVG GAAFDQFEHP LPPATLELAQ 

        70         80         90        100        110        120 
GSHAVLFGAV GDWKYDTLPR EFRPEQAILG LRKALGLFAN LRPAILYPEL ASASSLKPEI 

       130        140        150        160        170        180 
VSGLDILIIR ELTGDIYFGT PRGVRTAADG AFAGEREGYD TMRYAESEVR RIARIGFESA 

       190        200        210        220        230        240 
RKRNKKLCSV DKANVLETSQ FWRDLVIEVS RDYLDVELSH MYVDNAAMQL VRNPRQFDVI 

       250        260        270        280        290        300 
VTGNLFGDIL SDEAAMLTGS IGMLPSASLN AAGQGLYEPS HGSAPDIAGQ GIANPLATIL 

       310        320        330        340        350 
SAAMLLRYSL NLAPQADRVE AAVRKVLADG LRTADIHEAG TTKVSTSQMG DAVLKALG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX640415 Genomic DNA. Translation: CAE41772.1.
RefSeqNP_880220.1. NC_002929.2.

3D structure databases

ProteinModelPortalQ7VY73.
SMRQ7VY73. Positions 4-357.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2666599.
GenomeReviewsGene locus BP1483 in contig BX470248_GR.
KEGGbpe:BP1483.
NMPDRfig|257313.1.peg.1298.
PATRIC21156190. VBIBorPer7866_1591.

Phylogenomic databases

HOGENOMHBG518924.
OMAAAMMLRF.
PhylomeDBQ7VY73.
ProtClustDBPRK00772.

Enzyme and pathway databases

BioCycBPER257313:BP1483-MONOMER.

Family and domain databases

HAMAPMF_01033. LeuB_type1.
[Tree]
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KOK00052.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. LeuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU3_BORPE
AccessionPrimary (citable) accession number: Q7VY73
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: October 1, 2003
Last modified: January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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