Reviewed, UniProtKB/Swiss-Prot Q7VXY3 (RNPH_BORPE)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	BP1588

Organism

Bordetella pertussis [Complete proteome] [HAMAP]

Taxonomic identifier

520 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Ribonuclease PH HAMAP MF_00564		PRO_0000139874

Sequences

	Sequence		Length	Mass (Da)
	Q7VXY3-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: EFFDAA0AEBDEA567	FASTA	246	26,035
	10 20 30 40 50 60 MSTSAADTLS RPSGRAVDAL RPFSLERGFT RYAEGSVLVR AGNTHVLCTA SVLEKVPPFL 70 80 90 100 110 120 KGRGEGWVTA EYGMLPRATH TRGDREAARG KQSGRTQEIQ RLIGRSLRAV FDLRLLGERT 130 140 150 160 170 180 LHLDCDVLQA DGGTRCASIT GAWVAAADAV ALLMQRGDLA HNPIRDAVAA VSVGLVDGRA 190 200 210 220 230 240 VLDLDYQEDS ACAADVNVVM TGSGAFVEVQ GTGEGATFTR GELDTMLGLA EGGIAQLVRA QREALQ

References

[1]

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tohama I / ATCC BAA-589 / NCTC 13251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX640415 Genomic DNA. Translation: CAE41877.1.
RefSeq	NP_880321.1.
3D structure databases
SMR	Q7VXY3. Positions 9-245.
ModBase	Search...
Genome annotation databases
GeneID	2666618.
KEGG	bpe:BP1588.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	Q7VXY3.
Enzyme and pathway databases
BioCyc	BPER257313:BP1588-MONOMER.
BRENDA	2.7.7.56. 21511.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BORPE

Accession

Primary (citable) accession number: Q7VXY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)